NUCB2 Mouse mAb(Ab118866) - Western Blot All lanes: NUCB2 Mouse mAb(Ab118866) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 100.0 s
NUCB2 Mouse mAb(Ab118866) - IHC IHC-P analysis of stomach tissue by NUCB2 Mouse mAb(Ab118866). IHC-P was performed using sections of the formalin-fixed paraffin-embedded stomach tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NUCB2 Mouse mAb(Ab118866) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Glandular cells are positively stained at the cytoplasm.
NUCB2 Mouse mAb(Ab118866) - IP Immunoprecipitation was performed by incubation of 2.5 µg of NUCB2 Mouse mAb(Ab118866) with HL-60 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NUCB2 Mouse mAb(Ab118866) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was mouse IgG1. Lane 1: HL-60 lysate Lane 2: NUCB2 immunoprecipitated from HL-60 lysate by NUCB2 Mouse mAb(Ab118866) Lane 3: The same as Lane 2 was used as IgG isotype control antibody Result: NUCB2 Mouse mAb(Ab118866) can immunoprecipitate NUCB2.
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