PAK4 Mouse mAb (Ab120121) - Western Blot All lanes: PAK4 Mouse mAb (Ab120121) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 74, 75 kDa PAK4 can function as an antiapoptotic protein (PMID:14517283). And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822). Exposure time: 2.9 s
PAK4 Mouse mAb (Ab120121) - IP Immunoprecipitation was performed by incubation of 2.5 µg of PAK4 Mouse mAb (Ab120121) with HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PAK4 Mouse mAb (Ab120121) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: HEK293 lysate Lane 2: PAK4 immunoprecipitated from HEK293 lysate by PAK4 Mouse mAb (Ab120121) Lane 3: The same as Lane 2 but mouse IgG was used as IgG isotype control antibody Result: PAK4 Mouse mAb (Ab120121) can immunoprecipitate PAK4.
PAK4 Mouse mAb (Ab120121) - IHC IHC analysis of prostate tissue by PAK4 Mouse mAb (Ab120121). IHC was performed using sections of the formalin-fixed paraffin-embedded prostate tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PAK4 Mouse mAb (Ab120121) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Glandular cells are positively stained at the cytoplasm.
ZJ24F0910815
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