Mouse anti Human PCMT1 Antibody, Monoclonal (OTI2A9), could be used for WB, IHC and so on.
规格
产品名称
PCMT1 Mouse mAb
别名
PCMT1抗体 | PCMT1单克隆抗体 | 蛋白L-异天冬氨酸-O-甲基转移酶抗体
英文别名
L isoaspartyl protein carboxyl methyltransferase antibody | L isoaspartyl/D aspartyl methyltransferase antibody | L-isoaspartyl protein carboxyl methyltransferase antibody | PCMT 1 antibody | Pcmt1 antibody | PIMT antibody | PIMT_HUMAN antibody | Protein
规格或纯度
ExactAb™, 已验证, 见COA
生化机理
Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
宿主种属
小鼠(Mouse)
特异性
PCMT1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human PCMT1 (AA 60-285).
阳性对照
WB: HeLa, Jurkat, MCF7, K562, NIH/3T3 and C6 cell lysates. IHC: Human breast, colon, kidney, lung, pancreas tissues.
PCMT1 Mouse mAb (Ab120677) - Western Blot All lanes: PCMT1 Mouse mAb (Ab120677) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3.5 s
PCMT1 Mouse mAb (Ab120677) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human breast tissue tissue using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).
PCMT1 Mouse mAb (Ab120677) - IHC Immunohistochemical staining of paraffinembedded Human colon tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).
PCMT1 Mouse mAb (Ab120677) - IHC Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, Ab120677) (1/2000).
Mouse mAb (Ab120677) - IHC Immunohistochemical staining of paraffinembedded Human lung tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).
PCMT1 Mouse mAb (Ab120677) - IHC Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).