PECI/ECI2 Mouse mAb (Ab121138) - Western Blot All lanes: PECI/ECI2 Mouse mAb (Ab121138) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 39, 35 kDa PECI/ECI2 Mouse mAb (Ab121138) recognizes two isoforms of PECI/ECI2. Exposure time: 109.1 s
PECI/ECI2 Mouse mAb (Ab121138) - IP Immunoprecipitation was performed by incubation of 2.5 µg of PECI/ECI2 Mouse mAb (Ab121138) with Raji lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PECI/ECI2 Mouse mAb (Ab121138) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: Raji lysate Lane 2: ECI2 immunoprecipitated from Raji lysate by PECI/ECI2 Mouse mAb (Ab121138) Lane 3: The same as Lane 2 but mouse lgG was used as IgG isotype control antibody Result: PECI/ECI2 Mouse mAb (Ab121138) can immunoprecipitate ECI2.
PECI/ECI2 Mouse mAb (Ab121138) - IHC IHC analysis of liver tissue by PECI/ECI2 Mouse mAb (Ab121138). IHC was performed using sections of the formalin-fixed paraffin-embedded liver tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PECI/ECI2 Mouse mAb (Ab121138) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Hepatocytes are positively stained at the cytoplasm.
ZJ24F1215458
危险品化学品经营许可证（带存储）