Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) - Western Blot All lanes: Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 27 kDa Exposure time: 86.7 s
Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) - IHC Immunohistochemical analysis of PSMA5 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) - IF/ICC Immunofluorescent analysis of PSMA5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
ZJ24F0809406
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