Mouse anti Human RALB Antibody, Monoclonal (K92021_15B6), could be used for WB, IHC and so on. Application: WB: 1/1000 IHC: 1/2000 Protein Function: Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis.
规格
产品名称
RALB Mouse mAb
别名
RALB抗体 | Ras样蛋白A抗体 | RALB 小鼠单克隆抗体 | RALB 小鼠单抗
英文别名
V ral simian leukemia viral oncogene homolog B antibody | GTP binding protein | RalB | RAS-like protein B | ras-related protein Ral-B | v-ral simian leukemia viral oncogene homolog B (ras related | GTP bindingprotein) | 5730472O18Rik antibody | dRalb anti
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
小鼠(Mouse)
特异性
RALB
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant human RALB (AA 31-200).
阳性对照
WB: Hep G2, HEK293, A431, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.
RALB Mouse mAb (Ab124480) - Western Blot All lanes: RALB Mouse mAb (Ab124480) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 32.2 s
RALB Mouse mAb (Ab124480) - IHC IHC analysis of human testis tissue by RALB Mouse mAb (Ab124480). IHC was performed using sections of the formalin-fixed paraffin-embedded human testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RALB Mouse mAb (Ab124480) at 0.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Cells in seminiferous ducts are positively stained at the cytoplasm membrane.
ZJ24F0505557
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