Mouse anti Human RALBP1 Antibody, Monoclonal (OTI2A6), could be used for WB, IHC, IF/ICC, IP and so on.
规格
产品名称
RALBP1 Mouse mAb
别名
RALBP1抗体|RalA结合蛋白1抗体|RALBP1单克隆抗体
英文别名
RLIP1 antibody|76 kDa Ral-interacting protein antibody|76-kDa Ral-interacting protein antibody|Dinitrophenyl S-glutathione ATPase antibody|DNP-SG ATPase antibody|Ral-interacting protein 1 antibody|Ral-interacting protein 1, 76-KD antibody|RalA-binding protein 1 antibody|RalBP1 antibody|RLIP76 antibody|RALBP1|RIP1|RLIP1|RLIP76|RalBP1|Ral-interacting protein 1
规格或纯度
敲除验证, ExactAb™, 已验证, 见COA
生化机理
Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
宿主种属
小鼠(Mouse)
特异性
RALBP1
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant f ull length protein of human RALBP1.
阳性对照
WB: HeLa, Hep G2, MCF7, HT-29, NIH/3T3 and C6 cell lysates. IHC: Human breast adenocarcinoma, colon adenocarcinoma, kidney, ovary adenocarcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected
RALBP1 Mouse mAb (Ab124484) - Western Blot All lanes: RALBP1 Mouse mAb (Ab124484) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 76 kDa Observed band size: 91 kDa Exposure time: 60.0 s
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of breast tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of colon tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Kidney tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of ovary tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50)
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Adenocarcinoma of endometrium tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded prostate tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IHC Immunohistochemical staining of paraffinembedded Carcinoma of prostate tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).
RALBP1 Mouse mAb (Ab124484) - IF/ICC RALBP1 Mouse mAb (Ab124484) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.
RALBP1 Mouse mAb (Ab124484) - IF/ICC Immunofluorescent staining of Hep G2 cells using RALBP1 Mouse mAb (Ab124484).
RALBP1 Mouse mAb (Ab124484) - KO Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and RALBP1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with RALBP1 Mouse mAb (Ab124484) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
应用
应用名称
稀释比例
WB
1/1000
IF/ICC
1/100
IHC
1/50
IP
4 μg/mL
高级数据
关联靶点（人）
RALBP1TbioRalA 结合蛋白 1(RalA-binding protein 1)
(0 活性数据)
ZJ25F0117317
危险品化学品经营许可证（带存储）