Mouse anti Human RCAS1 Antibody, Monoclonal (K40051_4H7), could be used for WB, IHC and so on. Application: WB:1/1000 IHC:1/100 Protein Function: May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
RCAS1 Mouse mAb (Ab124859) - Western Blot All lanes: RCAS1 Mouse mAb (Ab124859) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 24 kDa Observed band size: 31 kDa Exposure time: 36.5 s
RCAS1 Mouse mAb (Ab124859) - IHC IHC analysis of human brain tissue by RCAS1 Mouse mAb (Ab124859). IHC was performed using sections of the formalin-fixed paraffin-embedded human brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RCAS1 Mouse mAb (Ab124859) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Glial cells and neuronal cells are positively stained at the cytoplasm.
ZJ24F0505560
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