S100 alpha 2/S100A2 Mouse mAb (Ab126175) - Western Blot All lanes: S100 alpha 2/S100A2 Mouse mAb (Ab126175) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouose IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 85.8 s
S100 alpha 2/S100A2 Mouse mAb (Ab126175) - IHC Immunohistochemical analysis of S100-A2 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with S100 alpha 2/S100A2 Mouse mAb (Ab126175) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
S100 alpha 2/S100A2 Mouse mAb (Ab126175) - IF/ICC Immunofluorescent analysis of S100-A2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with S100 alpha 2/S100A2 Mouse mAb (Ab126175) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
ZJ25F0116582
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