ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse anti Human SCAMP5 Antibody, Monoclonal (K06322_12G11), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/200
IP: 1/100
Protein Function
Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.
SCAMP5 Mouse mAb (Ab126689) - Western Blot
All lanes: SCAMP5 Mouse mAb (Ab126689) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 24 kDa
Exposure time: 100.0 s
SCAMP5 Mouse mAb (Ab126689) - IHC
IHC analysis of cerebellum tissue by SCAMP5 Mouse mAb (Ab126689).
IHC was performed using sections of the formalin-fixed paraffin-embedded cerebellum tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with SCAMP5 Mouse mAb (Ab126689) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cytoplasm of cells in granular layer and in molecular layer are positively stained.
SCAMP5 Mouse mAb (Ab126689) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of SCAMP5 Mouse mAb (Ab126689) with mouse brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. SCAMP5 Mouse mAb (Ab126689) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.
Lane 1: Mouse brain lysate
Lane 2: SCAMP5 immunoprecipitated from mouse brain lysate by SCAMP5 Mouse mAb (Ab126689)
Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody
Result: SCAMP5 Mouse mAb (Ab126689) can immunoprecipitate SCAMP5.
1/1000
1/100
1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1113688 |
分析证书 | Ab126689 |
| ZJ24F1012357 |
分析证书 | Ab126689 |
| ZJ24F1012356 |
分析证书 | Ab126689 |
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