Mouse anti Human TRIM5 alpha Antibody, Monoclonal (K94032_3D8), could be used for WB, IP and so on.
规格
产品名称
TRIM5 alpha Mouse mAb
别名
环指蛋白88抗体 | TRIM5 alpha抗体 | TRIM5 alpha单克隆抗体
英文别名
EC 6.3.2 | EC 6.3.2.- | RING finger protein 88 | RNF88 | RNF88tripartite motif protein TRIM | TRIM5 | TRIM5alpha | tripartite motif containing 5 | tripartite motif protein TRIM5 | tripartite motif-containing 5 | tripartite motif-containing protein 5 | RIN
规格或纯度
无载体, ExactAb™, 已验证, 1.0 mg/mL
生化机理
Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV) (PubMed:17156811). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 (PubMed:25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction (PubMed:25127057).
宿主种属
小鼠(Mouse)
特异性
TRIM5 alpha
种属反应性
人(Human)
免疫原
Recombinant protein of human TRIM5 (AA 31-270)
阳性对照
WB: Jurkat and HUVEC cell lysates. IP: Jurkat lysates.
TRIM5 alpha Mouse mAb (Ab132527) - Western Blot All lanes: TRIM5 alpha Mouse mAb (Ab132527) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 56 kDa Observed band size: 57 kDa Exposure time: 67.2 s
TRIM5 alpha Mouse mAb (Ab132527) - IP Immunoprecipitation was performed by incubation of 2.5 µg of TRIM5 alpha Mouse mAb (Ab132527) with Jurkat lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. TRIM5 alpha Mouse mAb (Ab132527) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: Jurkat lysate Lane 2: TRIM5 immunoprecipitated from Jurkat lysate by TRIM5 alpha Mouse mAb (Ab132527) Lane 3: The isotype control antibody instead of TRIM5 alpha Mouse mAb (Ab132527) in HeLa lysate. Result: TRIM5 alpha Mouse mAb (Ab132527) can immunoprecipitate TRIM5.
ZJ24F1216326
危险品化学品经营许可证（带存储）