hUbc12 antibody | hUbc12 antibody | NEDD8 carrier protein antibody | NEDD8 carrier protein antibody | NEDD8 conjugating enzyme Ubc12 antibody | NEDD8 conjugating enzyme Ubc12 antibody | NEDD8 protein ligase antibody | NEDD8 protein ligase antibody | NEDD8
规格或纯度
ExactAb™, 已验证, 见COA
生化机理
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
宿主种属
小鼠(Mouse)
特异性
UBE2M/UBC12
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),狗(Dog),猴(Monkey)
预测反应种属
牛(Cow)
免疫原
Recombinant full length protein of human UBE2M produced in HEK293T.
阳性对照
WB: Hep G2, HeLa, A549, HEK293, Ramos, NIH/3T3 and C6 cell lysates. IF/ICC: COS7 cells transfected by pCMV6-ENTRY UBE2M.
UBE2M/UBC12 Mouse mAb (Ab133254) - Western Blot All lanes: UBE2M/UBC12 Mouse mAb (Ab133254) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 20.0 s
UBE2M/UBC12 Mouse mAb (Ab133254) - IF/ICC UBE2M/UBC12 Mouse mAb (Ab133254) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.