Mouse anti Human USH1C/Harmonin Antibody, Monoclonal (K06328_5A8), could be used for WB, IHC and so on. Application: WB: 1/1000 IHC: 1/1000 Protein Function: May be involved in protein-protein interaction.
USH1C/Harmonin Mouse mAb (Ab133668) - Western Blot All lanes: USH1C/Harmonin Mouse mAb (Ab133668) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 62 kDa Observed band size: 67 kDa Exposure time: 100.0 s
USH1C/Harmonin Mouse mAb (Ab133668) - IHC IHC analysis of small intestine tissue by USH1C/Harmonin Mouse mAb (Ab133668).IHC was performed using sections of the formalin-fixed paraffin-embedded small intestine tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with USH1C/Harmonin Mouse mAb (Ab133668) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Glandular cells are positively stained at the cytoplasm and membrane.
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