Mouse anti Human VAMP8/EDB Antibody, Monoclonal (1414CT354.12.23.93), could be used for WB, IHC and so on. Application · WB: 1/1000-1/2000 · IHC: 1/100-1/500 Protein Function Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL
宿主种属
小鼠(Mouse)
特异性
VAMP8
种属反应性
人(Human)
免疫原
A KLH conjugated synthetic peptide from the N-terminal region of human VAMP8 (AA 2-24).
阳性对照
WB: THP-1 and K562 cell lysates. IHC: Human brain tissue.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
1414CT354.12.23.93
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 0.09% Sodium azide, pH 7.4.
防腐剂
0.09% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
VAMP8/EDB Mouse mAb (Ab133867) - Western Blot All lanes: VAMP8/EDB Mouse mAb (Ab133867) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 11 kDa Observed band size: 15 kDa Exposure time: 26.4 s
VAMP8/EDB Mouse mAb (Ab133867) - IHC Immunohistochemical analysis of paraffin-embedded H. brain section using VAMP8/EDB Mouse mAb (Ab133867) (N-term). VAMP8/EDB Mouse mAb (Ab133867) was diluted at 1/25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.