B2M | B2MG_HUMAN | Beta 2 microglobin | Beta 2 microglobulin | Beta 2 microglobulin precursor | Beta chain of mhc class 1 proteins | Beta chain of MHC class I molecules | Beta-2-microglobulin form pI 5.3 | CDABP0092 | Hdcma22p | IMD43 | beta-2-microglobul
规格或纯度
无载体,ExactAb™,无叠氮钠,已验证,高性能,PBS Only,1.0 mg/mL
宿主种属
小鼠(Mouse)
特异性
beta 2 Microglobulin
种属反应性
人(Human)
免疫原
Recombinant protein corresponding to Human beta-2-microglobulin
阳性对照
WB: HeLa, Raji, THP-1 and HepG2 Whole cell Lysates ICC/IF: HeLa cells
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
CD01/2B7
Format
Whole IgG
亚型
IgG2a
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 短期储存（1-2 周）。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。
图片
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - ICC/IF IF analysis of beta 2 Microglobulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta 2 Microglobulin Mouse mAb (Ab169199) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
ZJ24F0404430
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