Goat Anti-Bovine IgG H&L Antibody, Secondary antibody, could be used for WB, CLIA, LF, GICA, EIA and so on. WB: 1/500 -1/5000 CLIA: 0.5 -10µg/mL LF/ GICA: 0.5 - 2.0mg/mL EIA: 1/1000 - 1/50000
Goat Anti-Bovine IgG H&L Antibody (Ab176120) - ELISA Goat Anti-Bovine IgG H&L Antibody (Ab176120) was tested by ELISA. The plate was coated with 1.0 μg/mL Bovine IgG (Ab175960). Goat Anti-Bovine IgG H&L Antibody was biotinylated then added at a series of different dilution ratios. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA.
Goat Anti-Bovine IgG H&L Antibody (Ab176120) - ELISA Cross-reactivity of Goat Anti-Bovine IgG H&L Antibody (Ab176120) was tested by ELISA. The wells were coated with the indicated IgG standards at 1.0 µg/mL and incubated overnight at 4℃, followed by a 2% BSA blocking step for 2h at RT. Goat Anti-Bovine IgG H&L Antibody was biotinylated then added at different dilution ratio followed by incubation for 1h. Streptavidin-HRP (np156148) was used at 1/10000 dilution for this ELISA.