Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.
宿主种属
小鼠(Mouse)
特异性
MOB1B
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant full length protein of human MOBKL1A produced in HEK293T.
阳性对照
WB: HEK293T transfected with the pCMV6-ENTRY MOBKL1A cDNA for 48 hrs, HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 lysates. IHC: Human prostate carcinoma, prostate, liver and kidney tissues.
MOB1B Mouse mAb (Ab179614) - Western Blot All lanes: MOB1B Mouse mAb (Ab179614) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 100.0 s
MOB1B Mouse mAb (Ab179614) - Western Blot All lanes: MOB1B Mouse mAb (Ab179614) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 100.0 s
MOB1B Mouse mAb (Ab179614) - IHC Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)
MOB1B Mouse mAb (Ab179614) - IHC Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)
MOB1B Mouse mAb (Ab179614) - IHC Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)
MOB1B Mouse mAb (Ab179614) - IHC Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using MOB1B Mouse mAb (Ab179614). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab179614, Dilution 1/50)
ZJ25F0117344
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