Application Flow: 0.32 μg/mL –5 μg/mL ICC/IF: 0.5 μg/mL Mouse anti BrdU antibody, clone 1C6, is suitable for detecting incorporated BrdU in a wide variety of cell types.
BrdU Mouse mAb (Ab179916) - Flow Cytometry Dot plot showing BrdU-treated (A) and DMSO-treated (B) Jurkat cells stained with Ab179916. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature. Cells were stained with 1.25 μg/mL BrdU Mouse mAb (Ab179916) for 1 hour at room temperature. Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as a secondary antibody at a 1/1000 dilution. Propidium Iodide was used to stain total DNA.
BrdU Mouse mAb (Ab179916) - ICC/IF IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab179916) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab179916) at 0.5 μg/mL.
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).
Images were taken on the confocal laser scanning microscope.
ZJ23F1202283
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