增强 T 细胞对外来抗原的所有基本反应,即增殖、分泌淋巴因子、上调介导细胞-细胞相互作用的分子,以及有效帮助 B 细胞分泌抗体。对 T 细胞和 B 细胞之间的有效互动以及对 T 细胞依赖性抗原的正常抗体反应都至关重要。不会上调白细胞介素-2 的产生,但会促进白细胞介素-10 的合成。防止预激活 T 细胞凋亡。在 CD40 介导的免疫球蛋白类型转换中发挥关键作用。
宿主种属
亚美尼亚仓鼠(Armenian hamster)
特异性
ICOS
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Mouse T cell clone D10.G4.1
阳性对照
Flow: Human peripheral blood lymphocytes.
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
克隆类型
单克隆抗体
克隆号
C398.4A
Format
Whole IgG
亚型
Armenian Hamster IgG
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
未稀释的抗体溶液应保存在 2°C 至 8°C 之间,避免长时间暴露在光线下。切勿冷冻。
图片
CD278/ICOS Armenian Hamster mAb (PE) (Ab185169) - Flow Cytometry Human peripheral blood lymphocytes treated with 10 μg/mL PHA for 3 days are stained with CD278/ICOS Armenian Hamster mAb (PE) (Ab185169) (right panel) at 0.2μg/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (PE) (Ab180308)
CD278/ICOS Armenian Hamster mAb (PE) (Ab185169) - Flow Cytometry Human peripheral blood lymphocytes treated with 10 μg/mL PHA for 3 days are stained with CD278/ICOS Armenian Hamster mAb (PE) (Ab185169) (right panel) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody. Untreated lymphocytes were used as control (left panel).
应用
应用名称
稀释比例
Flow Cytometry
≤0.3 μg/test,
the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume