GFP/EGFP Mouse mAb (Ab186981) - Western Blot All lanes: GFP/EGFP Mouse Antibody (Ab186981) at 1/10000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 40 kDa, 54 kDa, 29kDa Observed band size: 41 kDa, 51 kDa, 30&27 kDa Exposure time: 21.2 s
GFP/EGFP Mouse mAb (Ab186981) - Western Blot All lanes: GFP/EGFP Mouse Antibody (Ab186981) at 1/10000 dilution Samples: Recombinant GFP Protein (rp156636) at 10 ng per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 28 kDa Observed band size: 29 kDa Exposure time: 79.2 s
GFP/EGFP Mouse Antibody (Ab186981) - IP All lanes: GFP/EGFP Mouse Antibody (Ab186981) at 1/5000 dilution Lane1: HEK-293T transfected with an empty vector, whole cell lysate Lane2: HEK-293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate Lane3: Anti-GFP Nanobody Magnetic Beads (G1373495) IP in HEK-293T transfected with an empty vector, whole cell lysate Lane4: Anti-GFP Nanobody Magnetic Beads (G1373495) IP in HEK-293T transfected with an empty vector containing an EGFP-myc-tag, whole cell lysate Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 27 kDa Observed band size: 27, 29 kDa Exposure time: 30.0 s