For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
规格
产品名称
MHC Class II Rat mAb (Biotin)
别名
组织相容性复合体β（HLA-DMB）抗体|MHC Class II抗体|生物素标记的抗MHC Class II抗体|MHC II 类大鼠 mAb（生物素）
英文别名
E-alpha-f|H-2 class II histocompatibility antigen, E-K alpha chain|H-2 class II histocompatibility antigen, E-U alpha chain|H-2E|Ia|Ia3|Ia-3|I-E|I-Ealpha|MHC class II (I-A/I-E)|MHC class II IA/IE|D6S221E antibody|DMA antibody|DMB antibody|DP beta 1 antibody|DP beta 1 chain antibody|DP(W4) beta chain antibody|DPB 1 antibody|DPB1 antibody|DRB antibody|H2Ea antibody|HLA class II histocompatibility antigen antibody|HLA class II histocompatibility antigen DM beta chain antibody|HLA class II histocompatibility antigen, DP beta 1 chain antibody|HLA class II histocompatibility antigen, DP(W4) beta chain antibody|HLA DMB antibody|HLA DP1A antibody|HLA DPB1 antibody|HLA SB alpha chain antibody|HLA-A antibody|HLA-A histocompatibility type antibody|HLA-DP antibody|HLA-DP histocompatibility type, beta-1 subunit antibody|HLA-DP1B antibody|HLA-DPB antibody|HLA-DPB1 antibody|HLADM antibody|HLADP1B antibody|HLASB antibody|HLASB histocompatibility type antibody|Human MHC class II HLA SB alpha antibody|LA class II histocompatibility antigen DP alpha 1 chain antibody|Major histocompatibility complex class II antibody|Major histocompatibility complex class II DP alpha 1 antibody|Major histocompatibility complex class II DP beta 1 antibody|Major histocompatibility complex, class I, A antibody|MHC class II antigen DMB antibody|MHC class II antigen DPB1 antibody|MHC class II DP3 alpha antibody|MHC class II DPA1 antibody|MHC class II HLA-DP-beta-1 antibody|MHC DPB1 antibody|MHC HLA DPB1 antibody|PLT1 antibody|Primed lymphocyte test 1 antibody|RING6 antibody|RING7 antibody|Biotin anti-mouse I-A/I-E Antibody|Biotin Rat Anti-Mouse I-A/I-E|MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Biotin|Biotin Anti-Mouse MHC II (I-A/I-E) Antibody[M5/114.15.2]|Biotin Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
宿主种属
大鼠(Rat)
特异性
MHC Class II
种属反应性
小鼠(Mouse)
免疫原
Activated C57BL/6 mouse spleen cells
阳性对照
Flow: Mouse splenocytes.
偶联
Biotin
克隆类型
单克隆抗体
克隆号
M5/114.15.2
Format
Whole IgG
亚型
Rat IgG2b
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
未稀释的抗体溶液应保存在 2°C 至 8°C 之间，避免长时间暴露在光线下。切勿冷冻。
图片
MHC Class II Rat mAb (Biotin) (Ab187210) - Flow Cytometry Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with MHC Class II Rat mAb (Biotin) (Ab187210) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG Isotype Control Antibody (Biotin) (Ab180310).
MHC Class II Rat mAb (Biotin) (Ab187210) - Flow Cytometry Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes cells (red) compared to an isotype control (blue). Cells were stained with MHC Class II Rat mAb (Biotin) (Ab187210) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
ZJ24F0809701
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