| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab213993-10μg (试用装) 申请此免费试用装（?） 作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！
|
10μg |
现货  |
| |
| Ab213993-25μg |
25μg |
现货 |
| |
| Ab213993-100μg |
100μg |
现货 |
|
| 产品名称 | Recombinant MHC Class II Antibody (AF555) |
|---|---|
| 别名 | 组织相容性复合体β（HLA-DMB）抗体 | MHC Class II抗体 |
| 英文别名 | D6S221E antibody | DMA antibody | DMB antibody | DP beta 1 antibody | DP beta 1 chain antibody | DP(W4) beta chain antibody | DPB 1 antibody | DPB1 antibody | DPB1_HUMAN antibody | DRB antibody | H2Ea antibody | HLA class II histocompatibility antigen ant |
| 规格或纯度 | ExactAb™, 已验证, 0.5 mg/mL |
| 生化机理 | Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading. |
| 宿主种属 | 大鼠(Rat) |
| 特异性 | MHC Class II |
| 种属反应性 | 小鼠(Mouse) |
| 免疫原 | Activated C57BL/6 mouse spleen cells |
| 阳性对照 | Flow: C57BL/6 mouse splenocytes |
| 偶联 | AF555 |
| Excitation(nm) | Green Laser(532nm) |
| Emission(nm) | 568nm |
| Ex/Em(nm) | Ex:553nm;Em:568nm |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 轻链亚型 | kappa |
| 纯化方法 | Protein A purified |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 0.5 mg/mL |
| 储存温度 | 2-8°C储存,避光,禁止冻结 |
| 运输条件 | 冰袋运输 |
| 稳定性与储存 | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| 分子类型 | 抗体 |
Recombinant MHC Class II Antibody (AF555) (Ab213993) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant MHC Class II Antibody (AF555) (Ab213993) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF555) (Ab211666).
Recombinant MHC Class II Antibody (AF555) (Ab213993) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant MHC Class II Antibody (AF555) (Ab213993) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF555) (Ab211666). Black - Unlabelled control, cells without incubation with primary antibody.
| 应用名称 | 稀释比例 |
|---|---|
| Flow Cytometry | ≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume. |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 日期 | 货号 |
|---|---|---|---|
 ZJ25F0318918 |
分析证书 | 25-03-07 | Ab213993 |
| ZJ25F0318917 |
分析证书 | 25-03-07 | Ab213993 |
| ZJ25F0318916 |
分析证书 | 25-03-07 | Ab213993 |
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