Mouse anti Human GBP6 Antibody, Monoclonal (K92016_6B11), could be used for WB, IHC and so on.
规格
产品名称
GBP6 Mouse mAb
别名
G蛋白结合蛋白6抗体 | GBP6抗体 | GBP6单克隆抗体
英文别名
GBP-6 | GTP-binding protein 6 | Guanine nucleotide-binding protein 6 | guanylate binding protein family, member 6 | Guanylate-binding protein 6
规格或纯度
ExactAb™, 无载体, 已验证, 1.0 mg/mL
生化机理
Interferon (IFN)-inducible GTPase that plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens, such as bacterial pathogens Listeria monocytogenes and Mycobacterium bovis BCG as well as the protozoan pathogen Toxoplasma gondii (By similarity).
Confers protection to several pathogens, including the bacterial pathogens Listeria monocytogenes and Mycobacterium bovis BCG as well as the protozoan pathogen Toxoplasma gondii (By similarity).
宿主种属
小鼠(Mouse)
特异性
GBP6
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant protein of human GBP6 (AA 291-570)
阳性对照
WB: Mouse liver, A431 lysates. IHC: Human esophagus tissue.
偶联
Unconjugated
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
150mM PBS, 0.09% Sodium azide, pH 7.4
防腐剂
0.09% Sodium azide
储存条件
-80℃储存,避免反复冻融
运输条件
特低温运输
稳定性与储存
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
GBP6 Mouse mAb (Ab191420) - Western Blot All lanes: GBP6 Mouse mAb (Ab191420) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 69 kDa Exposure time: 70.5 s
GBP6 Mouse mAb (Ab191420) - Western Blot All lanes: GBP6 Mouse mAb (Ab191420) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 69 kDa Exposure time: 60.0 s
GBP6 Mouse mAb (Ab191420) - IHC IHC analysis of esophagus tissue by GBP6 Mouse mAb (Ab191420). IHC was performed using sections of the formalin-fixed paraffin-embedded esophagus tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with GBP6 Mouse mAb (Ab191420) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Squamous epithelial cells are positively stained at the cytoplasm.
ZJ26F0636105
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