May function as a scaffold on which the coordinated assembly of proteins can occur. May play a role as an adapter that, via its PDZ domain, localizes LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Involved in both of the two fundamental mechanisms of bone formation, direct bone formation (e.g. embryonic flat bones mandible and cranium), and endochondral bone formation (e.g. embryonic long bone development). Plays a role during fracture repair. Involved in BMP6 signaling pathway.
宿主种属
兔(Rabbit)
特异性
PDLIM7
种属反应性
人(Human),大鼠(Rat)
免疫原
Recombinant protein of human PDLIM7 (AA 122-223).
阳性对照
WB: HeLa, U-2 OS and HSHC cell lysates. IF/ICC: Hep G2 cells. Flow Cytometry: Hep G2 cells.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
防腐剂
No
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃(12个月);收货后建议分装,避免反复冻融。
图片
Recombinant PDLIM7 Antibody (Ab337943) - IF/ICC
Immunofluorescent analysis of Hep G2 cells using Recombinant PDLIM7 Antibody (Ab337943) at 1/1000 dilution (red), and DAPI (blue).
Recombinant PDLIM7 Antibody (Ab337943) - Western Blot
All lanes: Recombinant PDLIM7 Antibody (Ab337943) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa
Exposure time: 51.3 s
Recombinant PDLIM7 Antibody (Ab337943) - Western Blot
Western blotting analysis using Recombinant PDLIM7 Antibody (Ab337943). PDLIM7 expression in wild-type (WT) and PDLIM7 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control.