计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
BAY-3827 is a potent and selective AMPK inhibitor with IC 50 values of 1.4 nM at low (10 µM ATP concentration) and 15 nM at high (2 mM ATP concentration). BAY-3827 shows over 500-fold selectivity for most of the 331 kinases. BAY-3827 prevents phosphorylation of acetyl-CoA carboxylase 1 and shows strongest anti-proliferative activity in androgen-dependent prostate cancer cell lines
In Vitro
BAY-3827 (0-200 μM) inhibits AMPK kinase activity with IC 50 values of 1.4 nM at low, 10 µM ATP concentration and 15 nM at high, 2 mM ATP concentration. BAY-3827 (0-200 μM) inhibits Aurora A, Flt3, c-Met and Rsk4 with IC 50 values of 1324, 124, 788 and 36 nM , respectively with 10 µM ATP concentration. BAY-3827 (overnight) strongly reduces ACC1 Ser79 phosphorylation in LNCaP and VCaP cells, and shows a lesser extent in IMR-32 and especially in Colo320 cells. BAY-3827 (0-10 nM; 6 d) shows strong inhibitory effects to LNCaP and VCaP cells. BAY-3827 (1 and 5 μM; 24 and 48 h) represses LIPE gene expression, reduces serine/threonine kinase AKT3 and blocks the expression of several genes from the mitochondrial carnitine palmitoyltransferase (CPT) family which is involved in acyl carnitine formation in VCaP cells. BAY-3827 (5 μM; 2-4 d) significantly increases the formation of lipid droplets in comparison to androgen treatment only. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: LNCaP, VCaP, 22Rv1, C4-2B, PC-3 and DU-145 prostate cancer cell lines Concentration: 0-10 nM Incubation Time: 6 d Result: Showed strong inhibitory effects for LNCaP and VCaP cells, two prostate cancer cell lines with IC 50 values of 0.28 and 1.71 nM, respectily. Inhibited proliferation of 22Rv1 cells with an IC 50 value of 5.55 nM.
Form:Solid
IC50& Target:IC50: 1.4 nM (AMPK kinase,10 µM ATP), 15 nM (AMPK kinase, 2 mM ATP), 1324 nM (Aurora A), 124 nM (Flt3), 788 nM (c-Met), 36 nM (Rsk4)
规格
规格或纯度
≥98%
英文名称
BAY-3827
生化机理
BAY-3827 是一种强效的选择性 AMPK 抑制剂，低浓度（10 µM ATP 浓度）时的 IC 50 值为 1.4 nM，高浓度（2 mM ATP 浓度）时为 15 nM。BAY-3827 对大多数 331 激酶的选择性超过 500 倍。BAY-3827 可防止磷酸化
储存条件
避光,-80℃储存
运输条件
特低温运输
作用类型
抑制剂
纯度
≥98%
名称和识别符
分子类型
小分子
分子量
468.53
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 25 mg/mL (53.36 mM; ultrasonic and warming and heat to 60°C)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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技术文档和文章
溶液计算器
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