计算溶液所需的质量、体积或浓度。
4-香豆酸辅酶A连接酶 (4CL)提取试剂
有货
为什么选择此级别
BioReagent,植物样品抽提用 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
4-香豆酸辅酶A连接酶是催化桂皮酸形成咖啡酸、香豆酸的酶，该酶多存在于高等植物、酵母、菌类可溶性部分物质，属于细胞木质素合成途径中间的关键酶，研究该酶可以探讨多种生物细胞发育过程中木质素沉积的代谢机理，为减少水果石细胞含量提高其品质提供依据。
4-香豆酸辅酶A连接酶 (4CL)提取试剂主要用于裂解植物组织，提取样品中的4-香豆酸辅酶A连接酶。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、蒸馏水
2、离心管或试管、匀浆器或研钵、低温离心机
操作步骤 (仅供参考)：
1、取植物组织清洗干净，切碎。
2、按植物组织：4-香豆酸辅酶A连接酶 (4CL)提取试剂=0.5g: 2ml的比例，加入4-香豆酸辅酶A连接酶 (4CL)提取试剂，冰浴情况下充分捣碎或研磨。
3、10000g，4℃离心15-20min，留取上清液。
4、-20℃冻存，用于4CL的检测或其他用途。
计算：
样品粗酶液获得率 (ml/g)=上清液体积 (ml)/样品质量 (g)×100%
注意事项：
1、待测样品中不能含有磷酸酶抑制剂，同时需避免反复冻融。
2、所测样本的值高于标准曲线的上限，应用提取试剂稀释样品后重新测定。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
4-Coumarate-CoA Ligase (4CL) is an enzyme that catalyzes the conversion of cinnamic acid into caffeic acid and coumaric acid. Widely distributed in the soluble fractions of higher plants, yeasts, and fungi, it acts as a key enzyme in the intracellular lignin biosynthesis pathway. Research on this enzyme can facilitate the exploration of metabolic mechanisms governing lignin deposition during the developmental processes of various biological cells, thereby providing a theoretical basis for reducing the content of stone cells in fruits and improving their quality.
The 4-Coumarate-CoA Ligase (4CL) Extraction Reagent is mainly used for lysing plant tissues and extracting 4CL from samples. This reagent is intended for research use only and is not suitable for clinical diagnosis or any other applications.
Materials to Be Prepared by the User
1. Distilled water
2. Centrifuge tubes or test tubes, homogenizer or mortar and pestle, refrigerated centrifuge
Operating Procedures (For Reference Only)
1. Take the plant tissue, rinse it thoroughly, and cut it into small pieces.
2. Add the 4-Coumarate-CoA Ligase (4CL) Extraction Reagent at a ratio of 0.5 g plant tissue: 2 ml reagent, then fully homogenize or grind the mixture under ice bath conditions.
3. Centrifuge at 10,000×g and 4°C for 15-20 minutes, and collect the supernatant.
4. Store the supernatant at -20°C for subsequent 4CL activity assays or other experimental applications.
Calculation
Yield of crude enzyme solution from samples (ml/g)=Volume of supernatant (ml)/Mass of sample (g)×100%
Precautions
1. The sample to be tested must not contain phosphatase inhibitors, and repeated freeze-thaw cycles should be avoided.
2. If the measured value of the sample exceeds the upper limit of the standard curve, dilute the sample with the extraction reagent and perform the assay again.
3. Please use the reagent as soon as possible after opening to prevent it from affecting the results of subsequent experiments.
4. For your safety and health, wear a lab coat and disposable gloves during the operation.
规格
别名
4-香豆酸辅酶A连接酶提取试剂 | 4CL提取试剂
英文别名
4-Coumarate-CoA Ligase Extraction Reagent | 4CL Extraction Reagent
规格或纯度
BioReagent, 植物样品抽提用
稳定性与储存
Store at 2-8℃ long term (12 months). Store in the dark.
英文名称
4-Coumarate-CoA Ligase (4CL) Extraction Reagent
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332519 |
分析证书 | C1509581 |
溶液计算器
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