计算溶液所需的质量、体积或浓度。
BioReagent,for western blot,无菌过滤 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
酪蛋白封闭缓冲液 (10×) 含高度纯化的酪蛋白分子及特有封闭因子,封闭性能优异,适用于饱和封闭印迹膜或其他固相表面,可有效降低背景、减小批内/批间变异系数(CV),提高检测灵敏度。本品亦可用于其他蛋白包被表面的封闭,兼容大多数检测系统。
产品基本信息
• 缓冲液:Tris
• pH值:7.2 ± 0.2
• 蛋白质:高度纯化的酪蛋白
• 防腐剂:0.0015%(w/v) 异噻唑啉酮
• 保质期:2年
• 储存温度:2–8°C
• 应用:Western blot(稀释为1×工作液后使用,用于封闭印迹膜、稀释抗体及酶标二抗。)
使用说明
酪蛋白封闭缓冲液 (10×) 为10倍浓缩液,使用前请用纯水按1份浓缩液 + 9份纯水(如100 mL + 900 mL)的比例稀释,充分混匀后即为1×工作液。为避免污染,建议现配现用。
Western blot 印迹膜封闭(示例):
1. 蛋白转印固定;
2. 封闭:加入适量1×酪蛋白封闭缓冲液,于20–37℃孵育一定时间(通常15–90分钟即可);也可于2–8℃封闭过夜,效果可能更佳;
3. 检测:按照实验步骤进行下一步操作。
注:一抗和二抗推荐用1×酪蛋白封闭缓冲液稀释,可获得更低的背景信号。
This buffer contains highly purified casein molecules and proprietary blocking factors, delivering superior blocking performance. It is designed for saturating blocking of blotting membranes and other solid-phase surfaces, effectively reducing background signals, lowering intra-assay and inter-assay coefficients of variation (CV), and enhancing detection sensitivity. The product is also suitable for blocking other protein-coated surfaces and compatible with most detection systems.
Basic Product Information
• Buffer: Tris
• pH: 7.2 ± 0.2
• Protein: Highly purified casein
• Preservative: 0.0015% (w/v) Isothiazolinone
• Shelf life: 2 years
• Storage temperature: 2–8°C
• Application: Western blot (Dilute to 1× working solution before use; suitable for blocking blotting membranes, diluting primary antibodies and enzyme-conjugated secondary antibodies.)
Instructions for Use
Casein Blocking Buffer (10×) is a 10-fold concentrated stock solution. Before use, dilute the concentrate with pure water at a ratio of 1 part concentrate to 9 parts pure water (e.g., 100 mL stock + 900 mL pure water). Mix thoroughly to prepare the 1× working solution. Prepare fresh working solution immediately before use to prevent contamination.
Protocol for Western Blot Membrane Blocking (Example):
1. Perform protein transfer and immobilization.
2. Blocking: Add an appropriate volume of 1× Casein Blocking Buffer. Incubate at 20–37 °C for a set duration (typically 15–90 minutes). Overnight blocking at 2–8 °C may yield improved results.
3. Detection: Proceed with subsequent experimental steps as required.
Note: Dilute primary and secondary antibodies with 1× Casein Blocking Buffer to achieve minimal background signals.
| pH | 7.0-7.4 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 | |
| 分析证书 | C485243 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Sterile-filtered 级别指南 → 查看 BioReagent 级别指南 → 查看 for Western blot 级别指南 →