B2526397计算溶液所需的质量、体积或浓度。
Cy2-SE (iodine)
有货
概述
Cy2-SE iodine is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance .
In Vitro
Protocol 1.Protein Preparetion 1) In order to obtain the best labeling effect, please prepare the protein (antibody) concentration as 2 mg/mL. 2) The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1 M sodium bicarbonate shall be used for adjustment. 3) If the protein concentration is lower than 2 mg/mL, the labeling efficiency will be greatly reduced. In order to obtain the best labeling efficiency, it is recommended that the final protein concentration range is 2-10 mg/mL. 4) The protein must be in the buffer without primary amine (such as Tris or glycine) and ammonium ion, otherwise the labeling efficiency will be affected. 2.Dye Preparation (Example for CY3-NHS ester) Add anhydrous DMSO into the vial of CY3-NHS ester to make a 10 mM stock solution. Mix well by pipetting or vortex. 3.Calculation of dye dosage The amount of CY3-NHS ester required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of CY3-NHS ester to protein is about 10. Example: assuming the required marker protein is 500 μL 2 mg/mL IgG (MW=150,000), use 100 μL DMSO dissolve 1 mg CY3-NHS ester, the required CY3-NHS ester volume is 5.05 μL, and the detailed calculation process is as follows: 1) mmol (IgG) = mg/mL (IgG) ×mL (IgG) / MW (IgG) =2 mg/mL × 0.5 mL / 150,000 mg/mmol= 6.7×10-6 mmol 2) mmol (CY3-NHS ester) = mmol (IgG) × 10 = 6.7×10-6 mmol×10 = 6.7 × 10-5 mmol 3) μL (CY3-NHS ester) = mmol (CY3-NHS ester) ×MW (CY3-NHS ester) / mg/μL (CY3-NHS ester) = 6.7 ×10-5 mmol ×753.88 mg/mmol / 0.01 mg/μL = 5.05 μL (CY3-NHS ester) 4.Run conjugation reaction 1) A good volume of freshly prepared 10 mg/mL CY3-NHS ester is slowly added to 0.5 mL protein sample In solution, gently shake to mix, then centrifuge briefly to collect the sample at the bottom of the reaction tube. Don'tmix well to prevent protein samples from denaturation and inactivation. 2) The reaction tubules were placed in a dark place and incubated gently at room temperature for 60 minutes at intervals.For 10-15 minutes, gently reverse the reaction tubules several times to fully mix the two reactants and raise the bar efficiency. 5.Purify the conjugation The following protocol is an example of dye-protein conjugate purification by using a SepHadex G-25 column. 1) Prepare SepHadex G-25 column according to the manufacture instruction. 2) Load the reaction mixture (From \Run conjugation reaction\) to the top of the SepHadex G-25 column. 3) Add PBS (pH 7.2-7.4) as soon as the sample runs just below the top resin surface. 4) Add more PBS (pH 7.2-7.4) to the desired sample to complete the column purification. Combine the fractions that contain the desired dye-protein conjugate. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
规格
别名
Cy2-SE（碘） | Cy2-SE 碘
英文别名
Cyanine2-SE | Cyanine2-SE (iodine)
规格或纯度
≥95%, Em:510nm;Ex:492nm
英文名称
Cy2-SE (iodine)
生化机理
Cy2-SE 碘是一种 CY 染料。CY 是 Cyanine 的缩写，是由两个氮原子通过奇数个甲基单元连接而成的化合物。氰基化合物具有波长长、吸收和发射可调、消光系数高的特点。
储存条件
避光,-20°C储存,干燥
运输条件
超低温运输
纯度
≥95%
名称和识别符
PubChem SID
分子类型
小分子
IUPAC Name
(2,5-dioxopyrrolidin-1-yl) 6-[2-[(E,3Z)-3-(3-ethyl-1,3-benzoxazol-2-ylidene)prop-1-enyl]-1,3-benzoxazol-3-ium-3-yl]hexanoate;iodide
INCHI
1S/C29H30N3O6.HI/c1-2-30-21-11-5-7-13-23(21)36-27(30)15-10-16-28-31(22-12-6-8-14-24(22)37-28)20-9-3-4-17-29(35)38-32-25(33)18-19-26(32)34;/h5-8,10-16H,2-4,9,17-20H2,1H3;1H/q+1;/p-1
InChi Key
RQYPAAVMQSMNBY-UHFFFAOYSA-M
Smiles
CCN1C2=CC=CC=C2OC1=CC=CC3=[N+](C4=CC=CC=C4O3)CCCCCC(=O)ON5C(=O)CCC5=O.[I-]
分子量
643.5
高级数据
系统学分类
分类树（Taxonomy Tree）
| 界（kingdom) | 有机化合物 |
|---|---|
| 超类（Superclass) | 有机杂环化合物 |
| 类（Class) | 苯并噁唑啉 |
| 亚类（Subclass） | 暂无 |
| 中间层级节点（Intermediate Tree Nodes） | 暂无 |
| 直接上位类(Direct Parent) | 苯并噁唑啉 |
| 其他上位类（Alternative Parents） | 苯并噁唑类 吡咯烷-2-酮 苯类化合物 噁唑类 二羧酰亚胺 杂芳族化合物 内酰胺类 乙烯酮缩醛 氧环化合物 氮杂环化合物 一元羧酸及其衍生物 胺类 碳氢化合物衍生物 羰基化合物 有机碘化物盐 有机氧化物 有机阳离子 |
| 分子骨架（Molecular Framework) | 芳香族杂多环化合物 |
| 取代基（Substituents） | 2-吡咯烷酮 - 氨基酸 - 芳香族杂多环化合物 - 氮杂环 - 吡唑 - 苯基化合物 - 苯并噁唑 - 苯并噁唑啉 - 羰基 - 羧酸衍生物 - 二羧酰亚胺 - 杂芳香族化合物 - 烃衍生物 - 酮烯缩醛或衍生物 - 肟 - 单羧酸或其衍生物 - 有机铵盐 - 有机碘化物盐 - 有机硝基化合物 - 有机氧化物 - 有机氧化合物 - 有机盐 - 有机氮化合物 - 有机氧化合物 - 氧杂环 - 噁唑 - 吡咯烷 - 吡咯烷酮 |
| 描述（Description） | 该化合物属于苯并噁唑烷类有机化合物。这类化合物由苯环与噁唑烷环共轭组成。噁唑烷为五元不饱和环状结构，1位和3位分别含有氮原子和氧原子，并具有两个双键。 |
| 外部描述符（External Descriptors） | 暂无 |
三维结构
交互式化学结构模型
化学和物理性质
溶解性
DMSO : 62.5 mg/mL (97.13 mM; ultrasonic and warming and heat to 60°C)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
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