计算溶液所需的质量、体积或浓度。
钙盐染色液 (改良茜素红S法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
钙在人体内大量存在，构成骨骼作为支持人体的支架，在分泌、运送、肌肉收缩、神经传导等也起重要作用。钙在机体内以两种形式存在，一种是离子钙，存在血液循环内，即所谓血钙；另一种是结合钙，和蛋白、碳酸或磷酸结合而沉着在组织内。除骨骼和牙齿外，正常时钙渗透在所有组织和细胞中，一般不以固体状态出现在组织内，但在某些情况下钙析出成固体并沉着于组织内，则为病理性钙盐沉着，沉着的钙盐主要是磷酸钙，其次为碳酸钙。钙盐通常是单折射的，但草酸钙是双折射的，当使用 HE 染色时钙一般呈紫蓝色。许多染料可以于钙形成螯合物，包括茜素红S、红紫素、核固红等。茜素红S是一种蒽醌类衍生物，是茜素磺酸钠盐，它能与碳酸钙或磷酸钙中的钙盐螯合形成橙红色复合物。一般来说这些染料在识别中至大量的钙时，效果优于轻微染色的微量钙沉积，但茜素红S往往对少量的沉积物可得到更可靠的结果。
钙盐染色常用方法有硝酸银法和茜素红S法，本染色液采用改良McGee-Russell法，用茜素红S和Mayer苏木素染色，尤其适用于少量钙盐组织的染色。
| C774863 | Component | 3×50mL | Storage |
| C774863A | 茜素红S染色液 | 50 mL | RT. Store in the dark. |
| C774863B | McGee-Russell分化液 | 50 mL | RT. Store in the dark. |
| C774863C | Mayer苏木素染色液 | 50 mL | 2-8℃. Store in the dark. |
使用方法
一、常规染色
1、组织固定于10%中性福尔马林固定液，常规脱水包埋。
2、切片厚5μm，常规脱蜡至水。
3、切片用茜素红S染色液滴染 1~5min（见注意事项 1），稍水洗。
4、McGee-Russell分化液迅速分化数秒。
5、Mayer苏木素染色液浅染胞核1~2min，流水冲洗10min。
6、常规脱水透明，中性树胶封固。
二、不溶性钙染色
1、组织固定于10%中性福尔马林固定液，常规脱水，注意在95%乙醇中脱水应充分。
2、塑料包埋，室温过夜，37°C烤箱中聚合2~4天，-20°C冰箱冷却15~20min。
3、切片厚度 3~5μm，未脱钙骨切片脱塑至水，蒸馏水洗。
4、切片入茜素红S染色液1~10min（见注意事项 2），蒸馏水洗。
5、（备选）McGee-Russell分化液迅速分化数秒。
6、（备选）Mayer苏木素染色液浅染胞核1~2min，流水冲洗10min。
7、常规脱水透明，中性树胶封固。
染色结果
| 钙沉积物 | 橙红色 |
| 细胞核 | 蓝色 |
注意事项
1、茜素红S染色时间要根据钙盐的含量来确定，可在显微镜下观察见钙盐呈较深的橙红色即取出水洗，如染色时间过长就出现弥散现象，一般1~2min即可。
2、在不溶性钙染色中，茜素红S的染色时间要根据钙盐的含量来确定，在病理性钙化及骨化的病理诊断及科研中常使用该法，如骨折的修复、骨化性肌炎、陈旧性瘢痕组织固化等。
3、McGee-Russell分化和Mayer苏木素染色不是必须步骤。
4、经过茜素红S染色液染色后，钙沉积物是双折射的。
5、茜素红S法在辨别和检测少量钙时特别有用，如检查肾中的异常钙化（尿钙过多）。
Calcium is abundant in the human body, forming bones that serve as the body's supporting framework, and also plays crucial roles in secretion, transport, muscle contraction, and nerve conduction. Calcium exists in the body in two forms: one is ionic calcium, present in the bloodstream (so-called blood calcium); the other is bound calcium, which combines with proteins, carbonate, or phosphate and deposits in tissues. Apart from bones and teeth, calcium normally permeates all tissues and cells and generally does not appear in a solid state within tissues. However, under certain pathological conditions, calcium can precipitate into solids and deposit within tissues, known as pathological calcification. The deposited calcium salts are primarily calcium phosphate, followed by calcium carbonate. Calcium salts are usually monorefringent, but calcium oxalate is birefringent. When stained with H&E, calcium generally appears purplish-blue. Many dyes can chelate with calcium, including Alizarin Red S, purpurin, nuclear fast red, etc. Alizarin Red S is an anthraquinone derivative, the sodium salt of alizarinsulfonic acid, which chelates with calcium salts in calcium carbonate or calcium phosphate to form an orange-red complex. Generally, these dyes are more effective in identifying moderate to large amounts of calcium than faintly stained micro-deposits, but Alizarin Red S often yields more reliable results for small amounts of deposits.
Common methods for calcium salt staining include the silver nitrate method and the Alizarin Red S method. This staining solution uses the modified McGee-Russell method with Alizarin Red S and Mayer's hematoxylin staining, particularly suitable for staining tissues with small amounts of calcium salts.
| C774863 | Component | 3×50mL | Storage |
| C774863A | Alizarin Red S Staining Solution | 50 mL | RT. Store in the dark. |
| C774863B | McGee-Russell Differentiation Solution | 50 mL | RT. Store in the dark. |
| C774863C | Mayer's Hematoxylin Staining Solution | 50 mL | 2-8℃. Store in the dark. |
Instructions for Use
I. Conventional Staining
Fix tissue in 10% neutral buffered formalin, followed by routine dehydration and embedding.
Cut sections at 5 µm thickness, and routinely deparaffinize to water.
Cover the section with Alizarin Red S Staining Solution and stain for 1~5 minutes (see Note 1). Rinse briefly with water.
Differentiate rapidly with McGee-Russell Differentiation Solution for a few seconds.
Counterstain nuclei lightly with Mayer's Hematoxylin Staining Solution for 1~2 minutes. Rinse under running tap water for 10 minutes.
Dehydrate and clear routinely, then mount with neutral balsam.
II. Insoluble Calcium Staining (e.g., for Undecalcified Bone)
Fix tissue in 10% neutral buffered formalin and dehydrate routinely. Ensure thorough dehydration in 95% ethanol.
Embed in plastic resin and polymerize overnight at room temperature, then in a 37°C oven for 2~4 days. Cool in a -20°C freezer for 15~20 minutes.
Cut sections at 3~5 µm thickness. Depolymerize undecalcified bone sections to water, then rinse with distilled water.
Immerse sections in Alizarin Red S Staining Solution for 1~10 minutes (see Note 2). Rinse with distilled water.
(Optional) Differentiate rapidly with McGee-Russell Differentiation Solution for a few seconds.
(Optional) Counterstain nuclei lightly with Mayer's Hematoxylin Staining Solution for 1~2 minutes. Rinse under running tap water for 10 minutes.
Dehydrate and clear routinely, then mount with neutral balsam.
Staining Results
| Calcium Deposits | Orange-red |
| Cell Nuclei | Blue |
Precautions
The staining time with Alizarin Red S should be determined based on the calcium salt content. Observe under a microscope and stop when calcium salts appear a deep orange-red, then rinse with water. Over-staining may cause diffusion; generally, 1~2 minutes is sufficient.
For insoluble calcium staining, the Alizarin Red S staining time should also be determined by calcium salt content. This method is commonly used in pathological diagnosis and research of pathological calcification and ossification, such as fracture repair, myositis ossificans, and calcification in old scar tissue.
Steps involving McGee-Russell differentiation and Mayer's hematoxylin counterstaining are not mandatory.
After staining with Alizarin Red S Staining Solution, calcium deposits are birefringent.
The Alizarin Red S method is particularly useful for identifying and detecting small amounts of calcium, such as checking for abnormal calcification in the kidney (nephrocalcinosis).
规格
别名
钙盐染色试剂盒
英文别名
Calcium Stain Kit (Modified Alizarin Red S Method)
规格或纯度
BioReagent,用于显微镜,生物染色剂
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Calcium Staining Solution (Modified Alizarin Red S Method)
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 I2607488 |
分析证书 | C774863 |
| ZJ26F0333468 |
分析证书 | C774863 |
技术文档和文章
Application Differences Among Highman, Bennhold, Puchtler, and Modified Methods in Congo Red Staining for Amyloid
目录： 技术文章
Application Differences Among Safranin O, Alcian Blue, and Toluidine Blue in Cartilage Matrix Staining
目录： 技术文章
Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining
目录： 技术文章
Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy
目录： 技术文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
选择需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）