LNCaP Whole Cell Lysate

货号: cl155902
有货
级别和纯度: 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 无叠氮钠 ? 无叠氮(Azide Free)—— 不含叠氮化钠防腐剂。适用于叠氮有毒或有抑制作用的偶联、细胞工作或检测。 Mycoplasma free
应用
WB
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规格
库存
价格
数量
200μg
cl155902-200μg
现货 Stock Image
¥2,269.90
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为什么选择此级别

无叠氮钠,无载体 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Product Description:

The LNCaP whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.

The LNCaP-FGC (fast growing colony) cell line is a subline derived from the LNCaP cell line, sharing all the main characteristics, including its androgen sensitivity, described for the parental line. These fibroblast-like cells express high levels of human prostatic acid phosphatase, androgen receptors and prostate antigen. They are used as a model of prostate cancer especially in studies of the development of androgen resistance in human prostate cancer.

规格

产品名称
LNCaP Whole Cell Lysate
别名
LNCaP 全细胞裂解物
规格或纯度
Carrier Free, Azide Free, Mycoplasma free
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
应用
WB
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail(EDTA-Free Protease Inhibitor Cocktail ) and phosphatase inhibitor(Na3VO4).
浓度
Mycoplasma free
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
图片

LNCaP Whole Cell Lysate (cl155902) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution


Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)


Lysates at 20 µg per lane


Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution


Predicted band size: 42 kDa
Observed band size: 42 kDa

LNCaP Whole Cell Lysate (cl155902) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution


Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)


Lysates at 20 µg per lane


Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution


Predicted band size: 29 kDa
Observed band size: 29 kDa

LNCaP Whole Cell Lysate (cl155902) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution


Lane 1: Jurkat Whole Cell Lysate (cl155897)
Lane 2: MOLT-4 Whole Cell Lysate (cl155905)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: Ramos Whole Cell Lysate (cl155908)


Lysates at 20 µg per lane


Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution


Predicted band size: 60 kDa
Observed band size: 58 kDa

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

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📊 产品数据表

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到1个结果

批号(Lot Number) 证书类型 货号
ZJ23F0700550 分析证书 cl155902
技术文档和文章
亚细胞组分分离方案
Subcellular Fractionation Protocols
蛋白质印迹通用实验方案
General Protocol for Western Blotting
蛋白质印迹的裂解物制备法
Methods of Lysate Preparation for Western Blotting
荧光标记抗体的蛋白质印迹法
Western Blotting with Fluorescently Labeled Antibodies
溶液计算器

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