The LNCaP whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
The LNCaP-FGC (fast growing colony) cell line is a subline derived from the LNCaP cell line, sharing all the main characteristics, including its androgen sensitivity, described for the parental line. These fibroblast-like cells express high levels of human prostatic acid phosphatase, androgen receptors and prostate antigen. They are used as a model of prostate cancer especially in studies of the development of androgen resistance in human prostate cancer.
规格
产品名称
LNCaP Whole Cell Lysate
别名
LNCaP 全细胞裂解物
规格或纯度
Carrier Free, Azide Free, Mycoplasma free
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
应用
WB
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail(EDTA-Free Protease Inhibitor Cocktail ) and phosphatase inhibitor(Na3VO4).
浓度
Mycoplasma free
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
图片
LNCaP Whole Cell Lysate (cl155902) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
LNCaP Whole Cell Lysate (cl155902) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
LNCaP Whole Cell Lysate (cl155902) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: Jurkat Whole Cell Lysate (cl155897) Lane 2: MOLT-4 Whole Cell Lysate (cl155905) Lane 3: LNCaP Whole Cell Lysate (cl155902) Lane 4: Ramos Whole Cell Lysate (cl155908)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa