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BioReagent,无菌,无菌过滤,用于细胞培养,通过内毒素测试 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Dulbecco的改良Eagle培养基—DMEM(Dulbecco's Modified Eagle Medium)是一种广泛使用的基础培养基,适用于多种哺乳动物细胞培养,包括原代成纤维细胞,神经元,神经胶质细胞,HUVEC和平滑肌细胞,以及HeLa,293,Cos-7和PC12等细胞系。DMEM是在MEM培养基的基础上研制的,与MEM培养基相比,氨基酸的含量增加了2倍,维生素增加了4倍,同时还增加了非必须氨基酸、微量铁离子以及丙酮酸钠。
DMEM培养基最初设计为葡萄糖含量1000mg/L的低糖型,后来又发展出葡萄糖含量为4500mg/L的高糖型,现已广泛应用于各种细胞的培养。DMEM高糖型普遍应用于生长快、粘附性低的细胞、杂交瘤的骨髓瘤细胞、克隆细胞、DNA转染的转化细胞、原代病毒宿主细胞、单一细胞的培养以及疫苗的生产,例如利用CHO细胞表达EPO和生产乙肝疫苗。
丙酮酸钠可以作为细胞培养中的替代碳源,在葡萄糖不足的情况下,细胞也可以代谢丙酮酸钠。不含丙酮酸钠的培养基,可以根据研究需求,自行调整丙酮酸钠的含量,以控制细胞代谢的碳源。HEPES是一种优良的生物缓冲剂,对细胞无毒性作用,培养基添加HEPES后能够较长时间保持恒定的PH范围,可以有效的防止培养液PH波动较大对细胞生长状态产生的不利影响。
本产品含有:D-葡萄糖(4500mg/L)、酚红、L-谷氨酰胺、HEPES。
不含有:丙酮酸钠。
Dulbecco’s Modified Eagle Medium (DMEM) is a widely used basal medium suitable for the culture of a broad range of mammalian cells, including primary fibroblasts, neurons, glial cells, human umbilical vein endothelial cells (HUVECs), smooth muscle cells, as well as established cell lines such as HeLa, 293, COS-7, and PC12 cells. DMEM was developed based on Minimum Essential Medium (MEM). Compared with MEM, DMEM contains approximately twice the concentration of amino acids and four times the concentration of vitamins, and is additionally supplemented with non-essential amino acids, trace amounts of iron, and sodium pyruvate.
DMEM was originally formulated as a low-glucose medium containing 1,000 mg/L D-glucose. A high-glucose formulation containing 4,500 mg/L D-glucose was subsequently developed and is now widely used for the culture of various cell types. High-glucose DMEM is commonly used for rapidly proliferating cells, cells with relatively low adhesion, myeloma cells used for hybridoma production, cloned cells, transformed cells generated by DNA transfection, primary cells used as viral host cells, single-cell cultures, and vaccine production. For example, it can be used for recombinant erythropoietin (EPO) expression in CHO cells and for hepatitis B vaccine production.
Sodium pyruvate can serve as an alternative carbon source in cell culture. When glucose availability is limited, cells can also metabolize sodium pyruvate. For media formulated without sodium pyruvate, its concentration can be adjusted according to specific experimental requirements, allowing control over the carbon sources available for cellular metabolism. HEPES is an effective biological buffer with low cytotoxicity. Supplementation of culture media with HEPES helps maintain a stable pH over extended periods and effectively reduces the adverse effects of pH fluctuations in the culture medium on cell growth.
This product contains: D-glucose (4,500 mg/L), phenol red, L-glutamine, and HEPES.
This product does not contain: sodium pyruvate.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | D1372047 | |
| 分析证书 | D1372047 |
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