Apo-D-amino acid oxidase is entirely present as a monomeric protein, while the reconstituted holoenzyme is a dimer of 79 kDa.
Contaminants: Free of FAD and Alkaline Phosphatase
规格
产品名称
D-氨基酸氧化酶
别名
D-α-氨基氧化酶 | D-氨基酸：氧气 氧化还原酶（脱氨）
英文别名
Apo D-Amino Acid Oxidase | Apo DAAO | D-amino-acid oxidase | D-AAO | D-Amino acid: oxygen oxidoreductase (deaminating) | DAO | DAAO | DAO1 | DAMOX
规格或纯度
生物活性,ActiBioPure™,天然,高性能,EnzymoPure™,≥80%,来源于猪肾脏; ≥25 U/mg protein
生化机理
Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Required to catabolize D-amino acids synthesized endogenously, of gastrointestinal bacterial origin or obtained from the diet, and to use these as nutrients (By similarity). Regulates the level of D-amino acid neurotransmitters in the brain, such as D-serine, a co-agonist of N-methyl D-aspartate (NMDA) receptors, and may modulate synaptic transmission (By similarity). Catalyzes the first step of the racemization of D-DOPA to L-DOPA, for possible use in an alternative dopamine biosynthesis pathway (By similarity). Also catalyzes the first step of the chiral inversion of N(gamma)-nitro-D-arginine methyl ester (D-NNA) to its L-enantiomer L-NNA that acts as a nitric oxide synthase inhibitor (By similarity). The hydrogen peroxide produced in the reaction provides protection against microbial infection; it contributes to the oxidative killing activity of phagocytic leukocytes and protects against bacterial colonization of the small intestine. Enzyme secreted into the lumen of the intestine may not be catalytically active and could instead be proteolytically cleaved into peptides with antimicrobial activity (By similarity). The hydrogen peroxide produced in the reaction may also play a role in promoting cellular senescence in response to DNA damage (By similarity). Could act as a detoxifying agent which removes D-amino acids accumulated during aging (By similarity). Miscellaneous: Variation of pH perturbs the enantioselectivity of the enzyme towards some amino acids. Catalytic activity: a D-alpha-amino acid + O2 + H2O = a 2-oxocarboxylate + H2O2 + NH4+13 Publications1 PublicationThis reaction proceeds in the forward direction.
Post-translational: Phosphorylated in the cerebellum; probably not by PRKACA, PRKCA or PRKCE. May be S-nitrosylated, which partially inactivates the enzyme.
生物活性
≥25 U/mg protein
种属
猪(Pig)
来源
天然
CAS编号和信息
9000-88-8
酶学委员会编号
EC 1.4.3.3
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
来源于猪肾脏; ≥25 U/mg protein
复溶
Reconstitute in distilled water or dilute buffer.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（24个月）；复溶后建议分装，避免反复冻融。
单位定义
The amount of enzyme that will deaminate by oxidation one micromole of D-alanine to pyruvate per minute at pH 8.3, at 37°C in the presence of catalase.
ZJ26F0435054
危险品化学品经营许可证（带存储）