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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
B- 细胞激活因子受体(BAFFR) 又称肿瘤坏死因子受体超家族成员 13C(TNFRSF13C)和 BLyS 受体 3(BR3),是 TNF 受体超家族的一种膜蛋白。B 细胞激活因子(BAFF)在体外能增强 B 细胞的存活率,是外周 B 细胞群的一个调节器。该基因所编码的蛋白是 BAFF 的受体,它含有一个细胞外的富含苯丙氨酸的结构域。该受体是 BAFF 介导的成熟 B 细胞存活所需的主要受体。在 B 细胞成熟过程中,由于受到 BAFF-R 的调节,只有有限的 B 细胞会存活。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人B-细胞激活因子受体(BAFFR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人B-细胞激活因子受体(BAFFR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human BAFFR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human BAFFR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.