BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Ⅰ型胶原蛋白（ColⅠ）是人体中含量最丰富的胶原蛋白，占比高达80%以上，是构成皮肤、骨骼、韧带、肌腱等结缔组织的主要成分。它具有两条α1链和一条α2链交错排列构成的三股螺旋结构，含有大量的甘氨酸、脯氨酸、羟脯氨酸。Ⅰ型胶原蛋白在细胞生长、分化、迁移和组织形态学发生等方面具有重要作用，具有优异的生物活性、机械强度和结构稳定性，能够提供力学支撑、促进伤口愈合、维持组织形态以及促进细胞生长。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人Ⅰ型胶原蛋白(ColⅠ)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Ⅰ型胶原蛋白(ColⅠ)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ColⅠ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ColⅠ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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