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抗十头肌麻痹同系物 7 或 SMAD7 的母亲是由 SMAD7 基因编码的蛋白质。顾名思义,它是果蝇基因的同系物:“抗五倍体麻痹症的母亲”,它属于 SMAD蛋白家族,属于 TGFβ配体超家族。与许多其他 TGFβ家族成员一样,SMAD7 参与细胞信号转导,它也是一种 TGFβ1 型受体拮抗剂。与 SMAD7 相关的疾病包括结直肠癌3 和乳腺癌。它的相关途径有乳腺癌综合途径和核SMAD2/3 信号的调节。与该基因相关的基因本体论注释包括 DNA 结合的转录因子活性和转录调节区 DNA 结合。该基因的一个重要同源物是 SMAD6。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人Smad同源物7(Smad7)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人Smad同源物7(Smad7)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human Smad7. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human Smad7 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.