BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Sirtuin（沉默交配型信息调节2同源物）5，又称SIRT5，是一种蛋白质，由SIRT5基因编码。该基因编码的是sirtuin家族的一个成员，与酵母的Sir2蛋白同源。sirtuin家族成员的特点是有一个sirtuin核心结构域，属于[组蛋白去乙酰化酶]超家族的第三类，并依赖NAD+作为酶活性的辅助因子。SIRT5是三个主要定位在线粒体的sirtuins之一。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人去乙酰化酶Sirtuin-5(SIRT5)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人去乙酰化酶Sirtuin-5(SIRT5)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SIRT5. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SIRT5 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
危险品化学品经营许可证（带存储）