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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素2(IL-2)是一个细胞因子家族的成员,其每个成员都有一个四条α螺旋束;该家族还包括IL-4、IL-7、IL-9、IL-15和IL-21。IL-2通过IL-2受体发出信号,这是一个由三条链组成的复合物,被称为α、β和γ。伽马链是所有家族成员共有的 。IL-2受体(IL-2R)α亚基与IL-2结合的亲和力低(Kd~ 10-8 M)。由于IL-2的细胞内链较短,IL-2和CD25单独相互作用不会导致信号转导,但有能力(当与β和γ亚单位结合时)将IL-2R的亲和力提高100倍。IL-2R的β和γ亚单位的异源二聚化对T细胞的信号传导至关重要。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白细胞介素2(IL-2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素2(IL-2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.