计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
由该基因编码的蛋白质是 TNF 受体超家族的成员。该受体优先在成熟 B 淋 巴细胞中表达,可能对 B 细胞发育和自身免疫反应很重要。该受体已显示 可 特 异 性 结 合 肿 瘤 坏 死 因 子 ( 配 体 ) 超 家 族 成 员 13b (TNFSF13B/TALL-1/BAFF) ,并导致 NF-kappaB 和 MAPK8/JNK 激活。该 受体还与各种 TRAF 家族成员结合,因此可以转导细胞存活和增殖的信号 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肿瘤坏死因子受体超家族成员17(TNFRSF17;BCMA)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肿瘤坏死因子受体超家族成员17(TNFRSF17;BCMA)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TNFRSF17;BCMA. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TNFRSF17;BCMA present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.