BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管内皮生长因子165，也被称为VEGF165，它是一种38kDa肝素结合同源二聚体糖蛋白，是分泌型可溶性蛋白，参与胚胎血管生成和血管生成。它可能由肿瘤细胞分泌，被认为在肿瘤血管生成过程中起重要作用。它与VEGFR-1和VEGFR-2具有高亲和力。VEGFR-1和VEGFR-2是受体酪氨酸激酶家族的成员。它是一种肝素结合生长因子，诱导HUVEC细胞的增殖，但不干扰VEGF121的活性。它能直接作用于血管内皮细胞促进血管内皮细胞增殖，增加血管通透性。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人血管内皮生长因子165(VEGF165)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人血管内皮生长因子165(VEGF165)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human VEGF165. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human VEGF165 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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