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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
趋化因子C-C-基元配体3样蛋白1(CCL3L1)是一种由CCL3L1基因编码的蛋白质。该基因是聚集在17号染色体q臂上的几个趋化因子基因之一。趋化因子是一个参与免疫调节和炎症过程的分泌蛋白家族。具体来说,趋化因子吸引淋巴细胞到感染或损伤的部位。该蛋白与几个趋化因子受体结合,包括趋化因子结合蛋白2(CCBP2或D6)和趋化因子(C-C图案)受体5(CCR5)。CCR5是HIV的一个共同受体,CCL3L1与CCR5的结合抑制了HIV的进入。此外,这种结合使受体通过内吞作用被带入细胞内,最终被重新处理和重新表达。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人趋化因子C-C-基元配体3样蛋白1(CCL3L1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人趋化因子C-C-基元配体3样蛋白1(CCL3L1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CCL3L1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CCL3L1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.