细菌鞭毛是细菌的运动器官，幽门螺杆菌能够从强酸性的胃内腔穿过胃上皮细胞上的黏液层达到胃上皮细胞的中性环境，这就是鞭毛运动作用的很好例证，通过鞭毛染色可以观察到鞭毛形态、数量和鞭毛在菌体分布的位置，鞭毛数量和在菌体上的分布位置是鉴定细菌的重要依据之一。
鞭毛染色的方法有改良Ryu法、石碳酸复红法、Leifson法、银染法、Loffler法、Cerares-Gill法等。阿拉丁鞭毛染色液(Cerares-Gill法)又称西萨-基尔染色法，主要适用于不易染色的细菌鞭毛、产生胶质较多的植物病原细菌以及鞭毛很细且容易脱落的细菌。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
F1510195
| Component
| 2×50ml
| Storage
|
F1510195A
| 媒染液A
| 25 mL
| RT
|
F1510195B
| 媒染液B
| 15mL
| RT
|
F1510195C
| 媒染液C
| 10 mL
| RT
|
F1510195D
| 品红复染液
| 50 mL
| RT. Store in the dark. |
临用前，取A、B、C按5:3:2充分混匀混合即为媒染液，染色时适当过滤后使用，根据染色情况可加50%～100%水稀释，不宜提前配制。
自备材料：
酒精灯、载玻片、接种环、显微镜、蒸馏水
操作步骤(仅供参考)：
1、细菌悬液涂片，加热固定或空气干燥固定。
2、滴加媒染液于涂片，处理3~5min。
3、流水冲洗去除媒染液。
4、滴加品红复染液处理3~5min。
5、水洗，空气干燥。
6、镜检：从涂片边缘开始，由外及里，逐渐移至中心；细菌分布少的地方，鞭毛容易观察；细菌密集的地方，鞭毛被菌体挡住，不易观察。
染色结果：菌体和鞭毛呈不同程度的红色。
注意事项：
1、玻片应洁净，无油污。
2、染色的菌种应连续传代多次，处于生长活跃期。
3、染色过程应小心操作，防止鞭毛脱落。
4、媒染液不宜久置，最好现用现配。
5、固定时不宜用高热火焰固定。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
Bacterial flagella are the motile organs of bacteria. Helicobacter pylori can pass through the mucus layer on gastric epithelial cells from the strongly acidic gastric lumen to reach the neutral environment of gastric epithelial cells, which is a good example of the motility function of flagella. Flagella staining allows the observation of flagellar morphology, quantity, and their distribution on bacterial cells. The number and distribution of flagella on bacterial cells are among the important criteria for bacterial identification.
Common flagella staining methods include the Modified Ryu Method, Carbol Fuchsin Method, Leifson Method, Silver Staining Method, Löffler Method, and Cerares-Gill Method. Aladdin Flagella Stain Solution (Cerares-Gill Method), also known as Cerares-Gill Staining Method, is mainly suitable for bacteria with flagella that are difficult to stain, plant pathogenic bacteria that produce large amounts of gelatinous substances, and bacteria with very thin flagella that are prone to detachment. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
Product Components and Storage Conditions:
F1510195
| Component
| 2×50ml
| Storage
|
F1510195A
| Mordant solution A
| 25 mL
| RT
|
F1510195B
| Mordant solution B
| 15mL
| RT
|
F1510195C
| Mordant solution C
| 10 mL
| RT
|
F1510195D
| Fuchsin counterstain solution
| 50 mL
| RT. Store in the dark. |
Immediately before use, mix reagents A, B and C thoroughly at a volume ratio of 5:3:2 to prepare the mordant solution. Filter the solution properly prior to staining, and dilute it with 50%–100% water depending on the staining results. Do not prepare the solution in advance.
Materials to Be Prepared:
Alcohol lamp, glass slide, inoculation loop, microscope, distilled water
Operating Procedures (For Reference Only):
1. Prepare a smear of the bacterial suspension, then fix it by heating or air drying.
2. Add mordant solution dropwise onto the smear and treat for 3–5 minutes.
3. Rinse the smear with running water to remove the mordant solution.
4. Add fuchsin counterstain solution dropwise and treat for 3–5 minutes.
5. Rinse with water and air dry.
6. Microscopic examination: Start from the edge of the smear and move gradually from the periphery to the center. Flagella are easier to observe in areas with sparse bacterial distribution; in densely populated bacterial areas, flagella are obscured by the bacterial cells and thus difficult to observe.
Staining Results:
Bacterial cells and flagella show varying degrees of red color.
Precautions:
1. Glass slides should be clean and free of oil stains.
2. The strains to be stained should be subcultured continuously for multiple generations and in the logarithmic growth phase.
3. Handle with care during the staining process to prevent flagella detachment.
4. The mordant solution should not be stored for a long time; it is best prepared fresh before use.
5. Avoid high-temperature flame fixation during the fixing step.
6. For your safety and health, wear a lab coat and disposable gloves during operation.
7. Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
ZJ26F0333094
危险品化学品经营许可证（带存储）