计算溶液所需的质量、体积或浓度。
鞭毛染色液 (改良Ryu法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
细菌鞭毛是细菌的运动器官，幽门螺杆菌能够从强酸性的胃内腔穿过胃上皮细胞上的黏液层达到胃上皮细胞的中性环境，这就是鞭毛运动作用的很好例证，通过鞭毛染色可以观察到鞭毛形态、数量和鞭毛在菌体分布的位置，鞭毛数量和在菌体上的分布位置是鉴定细菌的重要依据之一。
阿拉丁鞭毛染色液采用改良Ryu染色法，该染色法的优点是采用结晶紫作为核心染料，试剂比较稳定，操作简单，结果判断更可靠。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
蒸馏水、接种环、酒精灯、恒温箱、显微镜、载玻片
操作步骤(仅供参考)：
1、配制鞭毛染色工作液：使用前按试剂(A):试剂(B)=10:1混合，即为鞭毛染色工作液，室温保存待用。
2、在洁净无油脂的载玻片上滴加2滴蒸馏水。
3、用接种环挑取无菌蒸馏水，再与血平板上菌落接触，允许细菌游到接种环蒸馏水中，再将接种环移到玻片上蒸馏水顶部轻点2次。
4、轻轻摇动玻片，使细菌分布均匀，切勿研磨和搅动，以防鞭毛脱落。
5、置室温或35°C恒温箱内干燥固定。
6、滴加鞭毛染色工作液染色10～15min。
7、轻轻水洗，自然晾干。
8、镜检：从涂片边缘开始，由外及里，逐渐移至中心；细菌分布少的地方，鞭毛容易观察；细菌密集的地方，鞭毛被菌体挡住，不易观察。
染色结果：
菌体和鞭毛皆为紫色，菌体染色较鞭毛为深。
注意事项：
1、固定时不宜用火焰固定。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、试剂开封后请尽快使用，以防影响后续实验效果。
Bacterial flagella are the motile organs of bacteria. Helicobacter pylori can penetrate the mucus layer on gastric epithelial cells from the strongly acidic gastric lumen to reach the neutral microenvironment around the gastric epithelial cells, which is a good example of the motile function of flagella. Flagella staining allows the observation of flagellar morphology, quantity, and their distribution on bacterial cells. The number and distribution of flagella on bacterial cells are among the important criteria for bacterial identification.
Aladdin Flagella Stain Solution adopts the Modified Ryu Staining Method. The advantage of this staining method is that it uses crystal violet as the core dye, which makes the reagent relatively stable, the operation simple, and the result interpretation more reliable. This reagent is intended for research purposes only and is not suitable for clinical diagnosis or any other applications.
Materials to Be Prepared:
Distilled water, inoculation loop, alcohol lamp, incubator, microscope, glass slide
Operating Procedures (For Reference Only):
1. Prepare the flagella staining working solution: Mix Reagent (A) and Reagent (B) at a ratio of 10:1 before use to obtain the flagella staining working solution. Store it at room temperature for later use.
2. Place 2 drops of distilled water onto a clean, grease-free glass slide.
3. Use an inoculation loop to take a small amount of sterile distilled water, then touch the colonies on the blood agar plate to allow the bacteria to swim into the distilled water on the loop. Next, gently touch the top of the distilled water drops on the glass slide twice with the inoculation loop.
4. Shake the glass slide slightly to distribute the bacteria evenly; do not grind or stir vigorously to prevent flagella detachment.
5. Air-dry and fix the smear at room temperature or in a 35°C incubator.
6. Add the flagella staining working solution dropwise and stain for 10–15 minutes.
7. Rinse the slide gently with water and air-dry naturally.
8. Microscopic examination: Start from the edge of the smear and move gradually from the periphery to the center. Flagella are easier to observe in areas with sparse bacterial distribution; in densely populated bacterial areas, flagella are obscured by the bacterial cells and thus difficult to observe.
Staining Results:
Both bacterial cells and flagella stain purple, with the color of bacterial cells being deeper than that of flagella.
Precautions:
1. Avoid flame fixation during the fixing step.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
3. Use the reagent as soon as possible after opening to prevent it from affecting the results of subsequent experiments.
规格
别名
细菌鞭毛染色液 | 鞭毛染色液
英文别名
Flagellum Stain (Modified Ryu Method) | Bacterial Flagella Stain Solution
规格或纯度
BioReagent,生物染色剂,适用于微生物学,用于显微镜
英文名称
Flagellum Staining Solution (Modified Ryu Method)
应用
微生物染色
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
组分与储存
| F1510203 | Component | 100 mL | Storage |
| F1510203A | Ryu 稀释液 | 100 mL | RT. Store in the dark. |
| F1510203B | Ryu 染色液 | 10 mL | RT |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333151 |
分析证书 | F1510203 |
技术文档和文章
溶液计算器
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for microbiology 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）