游离脂肪酸（NEFA）主要由油酸、软脂酸、亚油酸等组成，血清浓度与脂类代谢、糖代谢及内分泌功能相关，亦可反映食品贮藏品质变化；本试剂盒采用酶偶联比色法：游离脂肪酸在ACS催化下生成乙酰辅酶A，经AOX氧化产生H₂O₂，再经POD催化与Trinder底物显色，于546 nm处测定吸光度即可定量NEFA含量。
Free fatty acids (NEFA) are mainly composed of oleic acid, palmitic acid, linoleic acid, and others. Their serum concentrations are associated with lipid metabolism, glucose metabolism, and endocrine function, and they also reflect changes in food storage quality. This kit employs an enzyme‑coupled colorimetric method: free fatty acids are converted to acetyl‑CoA by acyl‑CoA synthetase (ACS), which is then oxidized by acyl‑CoA oxidase (AOX) to produce H₂O₂. In the presence of peroxidase (POD), H₂O₂ reacts with a Trinder chromogenic substrate to produce a colored product. The NEFA content can be quantitatively determined by measuring the absorbance at 546 nm.
危险品化学品经营许可证（带存储）