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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酰胺合成酶（GS, EC 6.3.1.2）主要存在于植物中，是生物体内氨同化的关键酶之一，能够催化铵离子和谷氨酸合成谷氨酰胺，不仅可以防止过多的铵离子对生物有毒性，而且谷氨酰胺也是氨的主要储存和运输形式。GS 在ATP 和Mg²⁺存在时，催化铵离子和谷氨酸合成谷氨酰胺，谷氨酰胺进一步转化为γ-谷氨酰基异羟肟酸，在酸性条件下与铁形成的络合物在540nm处有最大吸收峰，可用分光光度计测定。
Glutamine Synthetase (GS, EC 6.3.1.2) is primarily found in plants and is one of the key enzymes involved in ammonia assimilation in living organisms. It catalyzes the synthesis of glutamine from ammonium ions and glutamate. This reaction not only prevents the toxicity caused by excessive ammonium ions but also allows glutamine to serve as a major storage and transport form of ammonia. In the presence of ATP and Mg²⁺, GS catalyzes the synthesis of glutamine from ammonium ions and glutamate. The glutamine formed is further converted into γ-glutamylhydroxamate, which, under acidic conditions, forms a colored complex with iron. This complex exhibits a maximum absorption peak at 540 nm, allowing its quantification by spectrophotometry.
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