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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
GS(EC 6.3.1.2)主要存在于植物中,是生物体内氨同化的关键酶之一,催化铵离子和谷氨酸合成谷氨酰胺,不仅可以防止过多的铵离子对生物有毒性,而且谷氨酰胺也是氨的主要储存和运输形式。 测定原理:GS在ATP和Mg²⁺存在下,催化铵离子和谷氨酸合成谷氨酰胺;谷氨酰胺进一步转化为γ─谷氨酰基异羟肟酸,在酸性条件下形成的络合物在540nm处有最大吸收峰,可用分光光度计测定。
Glutamine Synthetase (EC 6.3.1.2) is primarily found in plants and is one of the key enzymes for ammonia assimilation in living organisms. It catalyzes the synthesis of glutamine from ammonium ions and glutamate. This process not only prevents the toxicity caused by excessive ammonium ions but also allows glutamine to serve as a major form for ammonia storage and transport. Assay Principle: In the presence of ATP and Mg²⁺, GS catalyzes the synthesis of glutamine from ammonium ions and glutamate. The glutamine is then further converted to γ-glutamyl hydroxamate. Under acidic conditions, this forms a colored complex that exhibits a maximum absorption peak at 540 nm, which can be measured using a spectrophotometer.