计算溶液所需的质量、体积或浓度。
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货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
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G493781-1g |
1g |
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G493781-5g |
5g |
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G493781-25g |
25g |
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G493781-100g |
100g |
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产品名称 | β 半乳糖苷酶 来源于米曲霉 |
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规格或纯度 | EnzymoPure™, 150000u/g |
产品介绍 |
生化/生理作用: β-半乳糖苷酶作用于二糖、糖缀合物和多糖末端的β-D-半乳糖基。它可用于介导转糖基反应,比如低聚半乳糖(GOS)合成。ββ-半乳糖苷酶可用于糖生物学和生物技术应用。 β-半乳糖苷酶切割乳糖成单糖成分,葡萄糖和半乳糖。它还能催化葡萄糖转糖基化形成异乳糖,在反馈回路中作为β-半乳糖苷酶的诱导剂。 产品应用: β-半乳糖苷酶(来源于米曲霉Aspergillus oryzae)已用于:①体外核磁共振(NMR);②作为β-半乳糖苷酶分析标准品,测定细胞相关的β-半乳糖苷酶浓度; ③修饰突变滑液细胞(slime cell)表面的糖残基以研究粘附性;④乳糖酶促水解实验;⑤反相(RP)吸附实验;⑥乳清乳糖水解 产品特性: 1、温度范围:有效温度范围:20℃-70℃ 最适宜温度:40-60℃ 2、PH范围:有效PH范围:2.0-7.5 最适宜PH范围:4.0-5.5 酶学委员会编号:3.2.1.23 原理:乳糖酶能分解乳糖分子中的β-半乳糖苷键,生成葡萄糖和半乳糖。 酶活力定义:1g乳糖酶再PH4.5、37℃下,每分钟下分解ONPG(邻硝基苯β-D-半乳吡喃糖苷)产生1umol ONP(邻硝基酚)所需要的酶量,即1个酶活力单位以u/g表示。 Biochemical/physiological effects: β- Galactosidase acts on the ends of disaccharides, glycoconjugates and polysaccharides β- D-galactosyl. It can be used to mediate sugar transfer reactions, such as galactooligosaccharide (GOS) synthesis. ββ- Galactosidase can be used in glycobiology and biotechnology. β- Galactosidase cleaves lactose into monosaccharide components, glucose and galactose. It can also catalyze the transglycosylation of glucose to form isolactose, which acts as β- The inducer of galactosidase. Product application: β- Galactosidase (from Aspergillus oryzae) has been used for: ① in vitro nuclear magnetic resonance (NMR); ② As β- Galactosidase analysis standard, determination of cell related β- Galactosidase concentration; ③ To modify the sugar residues on the surface of the mutant synovial cells to study the adhesion; ④ Lactase hydrolysis experiment; ⑤ Reverse phase (RP) adsorption experiment; ⑥ Whey lactose hydrolysis Product characteristics: 1. Temperature range: effective temperature range: 20 ℃ - 70 ℃ Optimum temperature: 40-60 ℃ 2. PH range: effective PH range: 2.0-7.5 Optimum PH range: 4.0-5.5 Enzymology Committee No.: 3.2.1.23 Principle: lactase can decompose β- A galactoside bond that produces glucose and galactose. Definition of enzyme activity: 1g lactase decomposes ONPG (o-nitrobenzene) every minute at PH 4.5 and 37 ℃ β- D-galactopyranoside) to produce 1umol ONP (o-nitrophenol), that is, one enzyme activity unit is expressed in u/g. |
Accession # | P48825 |
Nacres | NA.54 |
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活性类型 | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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浓度 | 150000u/g |
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储存温度 | -20°C储存 |
运输条件 | 超低温冰袋运输 |
CAS编号和信息 | 9031-11-2 |
分子类型 | 小分子 |
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批号(Lot Number) | 证书类型 | 日期 | 货号 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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分析证书 | 22-11-19 | G493781 |
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1. Wang Jiawei, Du Yi, Du Jianxiu. (2022) Green-emission nitrogen-doped carbon quantum dots from alkaline N-methyl-2-pyrrolidinone for determination of β-galactosidase and its inhibitors. MICROCHIMICA ACTA, 189 (8): (1-10). [PMID:35840722] [10.1007/s00604-022-05378-3] |
1. Wang Jiawei, Du Yi, Du Jianxiu. (2022) Green-emission nitrogen-doped carbon quantum dots from alkaline N-methyl-2-pyrrolidinone for determination of β-galactosidase and its inhibitors. MICROCHIMICA ACTA, 189 (8): (1-10). [PMID:35840722] [10.1007/s00604-022-05378-3] |