计算溶液所需的质量、体积或浓度。
| 活性类型 | Relation | Activity value | Units | Action Type | 期刊 | PubMed Id | doi | Assay Aladdin ID |
|---|
EnzymoPure™ 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 3 篇同行评审文献引用。
生化/生理作用:
β-半乳糖苷酶作用于二糖、糖缀合物和多糖末端的β-D-半乳糖基。它可用于介导转糖基反应,比如低聚半乳糖(GOS)合成。ββ-半乳糖苷酶可用于糖生物学和生物技术应用。
β-半乳糖苷酶切割乳糖成单糖成分,葡萄糖和半乳糖。它还能催化葡萄糖转糖基化形成异乳糖,在反馈回路中作为β-半乳糖苷酶的诱导剂。
产品应用:
β-半乳糖苷酶(来源于米曲霉Aspergillus oryzae)已用于:①体外核磁共振(NMR);②作为β-半乳糖苷酶分析标准品,测定细胞相关的β-半乳糖苷酶浓度;
③修饰突变滑液细胞(slime cell)表面的糖残基以研究粘附性;④乳糖酶促水解实验;⑤反相(RP)吸附实验;⑥乳清乳糖水解
产品特性:
1、温度范围:有效温度范围:20℃-70℃
最适宜温度:40-60℃
2、PH范围:有效PH范围:2.0-7.5
最适宜PH范围:4.0-5.5
酶学委员会编号:3.2.1.23
原理:乳糖酶能分解乳糖分子中的β-半乳糖苷键,生成葡萄糖和半乳糖。
酶活力定义:1g乳糖酶再PH4.5、37℃下,每分钟下分解ONPG(邻硝基苯β-D-半乳吡喃糖苷)产生1umol ONP(邻硝基酚)所需要的酶量,即1个酶活力单位以u/g表示。
Biochemical/physiological effects:
β- Galactosidase acts on the ends of disaccharides, glycoconjugates and polysaccharides β- D-galactosyl. It can be used to mediate sugar transfer reactions, such as galactooligosaccharide (GOS) synthesis. ββ- Galactosidase can be used in glycobiology and biotechnology.
β- Galactosidase cleaves lactose into monosaccharide components, glucose and galactose. It can also catalyze the transglycosylation of glucose to form isolactose, which acts as β- The inducer of galactosidase.
Product application:
β- Galactosidase (from Aspergillus oryzae) has been used for: ① in vitro nuclear magnetic resonance (NMR); ② As β- Galactosidase analysis standard, determination of cell related β- Galactosidase concentration;
③ To modify the sugar residues on the surface of the mutant synovial cells to study the adhesion; ④ Lactase hydrolysis experiment; ⑤ Reverse phase (RP) adsorption experiment; ⑥ Whey lactose hydrolysis
Product characteristics:
1. Temperature range: effective temperature range: 20 ℃ - 70 ℃
Optimum temperature: 40-60 ℃
2. PH range: effective PH range: 2.0-7.5
Optimum PH range: 4.0-5.5
Enzymology Committee No.: 3.2.1.23
Principle: lactase can decompose β- A galactoside bond that produces glucose and galactose.
Definition of enzyme activity: 1g lactase decomposes ONPG (o-nitrobenzene) every minute at PH 4.5 and 37 ℃ β- D-galactopyranoside) to produce 1umol ONP (o-nitrophenol), that is, one enzyme activity unit is expressed in u/g.
| Nacres | NA.54 |
|---|
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 | |
| 分析证书 | G493781 |