J2520648计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 74 篇同行评审文献引用。
概述
人胰岛素溶液可作为添加剂用于Dulbecco′改良eagle′培养基(DMEM)培养上皮细胞系和人子宮內膜细胞系，用于最低必需培养基培养人乳腺癌细胞系，用于培养基199培养正常卵巢细胞系。人胰岛素溶液用于细胞培养的浓度建议为0.5-1 mL/L培养基。人胰岛素溶液可用于小鼠和大鼠的胰岛素耐受试验。它还可用于人胚胎干细胞（hESC）的体外培养和hMSC（人间充质基质细胞）的成脂分化。应用：
胰岛素是由胰岛β-细胞分泌的双链多肽激素。其分子量为~5800 Da。α链和β链是由两个链间二硫键所连接的。α链含有一个链间二硫键。
使用方法
重组人胰岛素溶液常添加至培养基中用于细胞培养，使用培养基稀释到目的浓度即可。也可用无菌水或PBS稀释。1. 根据实验需求，将重组人胰岛素溶液稀释到所需的工作浓度。许多细胞培养基中胰岛素的常用浓度为1-10 μg/mL，主要根据细胞类型来决定。2. 将稀释好的溶液根据目的浓度添加到培养基中。例如，如果你需要在100 mL培养基中添加5 μg/mL的胰岛素，可以加入500 μL的10 μg/mL胰岛素溶液。
注意事项
1. 本产品经0.22 μm过滤除菌，使用本产品时应注意无菌操作，避免污染；
2. 本产品为10 mg/mL浓缩液，请根据需要稀释后使用；
3. 2-8℃中解冻，混匀后使用，切忌反复冻融，用量较少时建议分装冻存，2-8℃中保存，至少6个月有效；
4. 不宜长时间放置于室温或较高温度的环境中；
5. 溶解后若有沉淀产生，待溶液升至室温后混匀即可溶解；若遇无法溶解时，用无菌的0.1 M HCl缓慢调节PH至2-3之间（边调节，边用pH试纸检测pH）；若仍然无法溶解，请及时与我们联系；
6. 本产品仅用于科研或进一步研究使用，不用于诊断和治疗。
Insulin is a two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond.
Application
Insulin solution human has been used as a supplement in Dulbecco′s modified eagle′s medium (DMEM) to culture epithelial cell line and human endometrial cell line, minimum essential medium eagle to culture human breast adenocarcinoma cell line and medium 199 to culture normal ovarian cell line.
Insulin solution human is recommended for use in cell culture applications at 0.5 to 1mL per liter of medium.
Insulin solution human has been used for insulin tolerance test in mice and rats. It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).
Usage
Recombinant human insulin solution is often added to the medium for cell culture, and the medium is diluted to the target concentration. It can also be diluted with sterile water or PBS. 1.According to the experimental requirements, the recombinant human insulin solution was diluted to the required working concentration. The common concentration of insulin in many cell cultures is 1-10 μg / mL, which is mainly determined by cell type. 2.Add the diluted solution to the medium according to the target concentration. For example, if you need to add 5 μg / mL insulin to 100 mL medium, 500 μL of 10 μg / mL insulin solution can be added.
Announcement
1.This product is filtered and sterilized by 0.22μm.When using this product, attention should be paid to aseptic operation to avoid pollution ;
2.This product is 10 mg / mL concentrate, please use it after dilution as needed ;
3.2-8 °C thawed, mixed well after use, avoid repeated freezing and thawing, less dosage is recommended subpackage cryopreservation, 2-8 °C preservation, at least 6 months effective ;
4.It should not be placed at room temperature or higher temperature for a long time ;
5.If there is precipitation after dissolution, the solution can be dissolved by mixing after the solution is raised to room temperature ; if it cannot be dissolved, use sterile 0.1 M HCl to slowly adjust the pH to between 2-3 ( while adjusting, while using the pH test paper to detect pH ) ; if still unable to dissolve, please contact us in time ;
6.This product is only used for scientific research or further research, not for diagnosis and treatment.
Insulin solution human has been used for insulin tolerance test in mice and rats. It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).
Usage
Recombinant human insulin solution is often added to the medium for cell culture, and the medium is diluted to the target concentration. It can also be diluted with sterile water or PBS. 1.According to the experimental requirements, the recombinant human insulin solution was diluted to the required working concentration. The common concentration of insulin in many cell cultures is 1-10 μg / mL, which is mainly determined by cell type. 2.Add the diluted solution to the medium according to the target concentration. For example, if you need to add 5 μg / mL insulin to 100 mL medium, 500 μL of 10 μg / mL insulin solution can be added.
Announcement
1.This product is filtered and sterilized by 0.22μm.When using this product, attention should be paid to aseptic operation to avoid pollution ;
2.This product is 10 mg / mL concentrate, please use it after dilution as needed ;
3.2-8 °C thawed, mixed well after use, avoid repeated freezing and thawing, less dosage is recommended subpackage cryopreservation, 2-8 °C preservation, at least 6 months effective ;
4.It should not be placed at room temperature or higher temperature for a long time ;
5.If there is precipitation after dissolution, the solution can be dissolved by mixing after the solution is raised to room temperature ; if it cannot be dissolved, use sterile 0.1 M HCl to slowly adjust the pH to between 2-3 ( while adjusting, while using the pH test paper to detect pH ) ; if still unable to dissolve, please contact us in time ;
6.This product is only used for scientific research or further research, not for diagnosis and treatment.
规格
产品名称
胰岛素 溶液 人
别名
重组人胰岛素溶液
规格或纯度
BioReagent, 10mg/ml
生化机理
胰岛素在各种细胞过程中起作用，包括促进细胞生长、调节细胞周期、促进葡萄糖摄取。常用作哺乳动物细胞无血清培养基的蛋白添加剂，促进并维持哺乳动物细胞的最优生长。
CAS编号和信息
11061-68-0
分子类型
未知
储存与运输
物理形态
液体
浓度
10mg/ml
储存条件
避光,-20°C储存
运输条件
超低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
Selection Strategies for Animal-Derived Serum, Functionalized Serum, and Non-Animal-Derived Culture Supplementation Systems in Cell Culture
目录： 技术文章
糖尿病动物模型建立与评价方法
目录： 技术文章
Applications of Endocrinology/Hormones in Metabolic Regulation, Reproductive Function, and Disease Research
目录： 技术文章
此产品的引用文献
溶液计算器
常见问题
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于?20 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 11061-68-0。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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