BioReagent 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 5 篇同行评审文献引用。
α-乳清蛋白可结合多种金属离子，包括钙，这一性质被认为在从还原的变性形式再生成天然α-乳清蛋白的过程中发挥重要作用。α-乳清蛋白还具有独特的锌结合位点，其被认为在乳糖合酶复合物的结合中发挥作用。成熟蛋白质由123个氨基酸残基（14kD）组成，并且具有1.7 Α°分辨率三维结构，含四个α-螺旋和三链反式平行β-折叠。其可通过改变半乳糖基转移酶的底物特异性以增加乳糖形成的速率；半乳糖基转移酶和α-乳白蛋白的复合物称为乳糖合酶。Asp87 或 Asp88 对 Ala 的定点诱变完全消除了强钙结合亲和力，并将乳糖合酶的刺激降低到最大速率的 <3.5%。
应用：
α-从牛乳中提取的乳清蛋白作为补充剂，用于各种细胞培养的基础培养基。也已作为标记物，用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）。
产品参数
| 项目 | 规格 |
| 产品名称 | α-乳白蛋白 |
| Ca(mol/mol) | 0.3-1.0 |
| CAS号 | 9051-29-0 |
| 来源 | 牛乳 |
| 分子量 | 14kDa（还原条件下） |
| 纯度 | ≥95%（SDS-PAGE） |
| 外观 | 白色冻干粉末 |
| 储存条件 | -20℃保存 |
α‑Lactalbumin can bind multiple metal ions, including calcium, and this property is considered to play an important role in the regeneration of the native protein from its reduced denatured form. α‑Lactalbumin also possesses a unique zinc‑binding site, which is thought to be involved in the binding of the lactose synthase complex. The mature protein consists of 123 amino acid residues (14 kDa) and has a three‑dimensional structure at 1.7 Å resolution, containing four α‑helices and a three‑stranded antiparallel β‑sheet. It can increase the rate of lactose formation by altering the substrate specificity of galactosyltransferase; the complex of galactosyltransferase and α‑lactalbumin is termed lactose synthase. Site‑directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the high‑affinity calcium binding and reduces the stimulation of lactose synthase to <3.5 % of the maximum rate.
Applications:
α‑Lactalbumin extracted from bovine milk is used as a supplement in basal media for various cell cultures. It is also used as a marker for sodium dodecyl sulfate‑polyacrylamide gel electrophoresis (SDS‑PAGE).
| Item | Specification |
|---|---|
| Product Name | α-Lactalbumin |
| Ca (mol/mol) | 0.3-1.0 |
| CAS No. | 9051-29-0 |
| Source | Bovine milk |
| Molecular Weight | 14 kDa (under reducing conditions) |
| Purity | ≥95% (SDS-PAGE) |
| Appearance | White lyophilized powder |
| Storage Condition | Store at -20°C |
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