碳酸锂 (Lithium carbonate) 分子式为 Li₂CO₃ ,分子量为73.89,CAS号为554-13-2。可以作为某些化学反应的催化剂,临床上亦有抗躁狂作用。HE染色或其他组织染色后的返蓝很重要,苏木素在酸性条件下处于红色离子状态,呈红色:在碱性条件下处于蓝色离子状态,呈蓝色。组织切片经盐酸乙醇分化后呈红色或粉红色,故分化之后,立即用水除去组织切片上的酸而中止分化,再用弱碱性水使苏木素染.上的细胞核呈现蓝色,这个过程称为返蓝作用或蓝化作用。
碳酸锂水溶液 (0.05%)主要由碳酸锂、去离子水组成,经常用于HE染色或其他组织染色后的返蓝,是一种非常重要的辅助试剂。
操作步骤(仅供参考):
1、根据实验具体要求操作,蓝化后应立即用水冲洗干净。
2、一般蓝化3-30s不等,应根据具体组织和切片厚度调整时间。
注意事项:
1、密闭保存,一旦开启尽快用完,因为其有效成分易挥发。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
3、本产品仅由于科研,严禁他用。
Lithium carbonate has a molecular formula of Li₂CO₃, a molecular weight of 73.89, and a CAS number of 554-13-2. It can act as a catalyst in certain chemical reactions and also has an antimanic effect in clinical settings. Bluing (or bluing reaction) after HE staining or other tissue staining is of great importance. Hematoxylin exists in a red ionic state and appears red under acidic conditions; under alkaline conditions, it exists in a blue ionic state and appears blue. Tissue sections turn red or pink after differentiation with hydrochloric acid-ethanol. Therefore, immediately after differentiation, water should be used to remove the acid from the tissue sections to terminate the differentiation process. Then, weakly alkaline water is used to make the cell nuclei stained with hematoxylin appear blue. This process is called bluing or bluing reaction.
Lithium Carbonate Aqueous Solution (0.05%) is mainly composed of lithium carbonate and deionized water. It is often used for bluing after HE staining or other tissue staining, and serves as a very important auxiliary reagent.
Operating Procedures (for Reference Only)
1. perate in accordance with the specific requirements of the experiment. After bluing, the sections should be rinsed thoroughly with water immediately.
2. Generally, the bluing time ranges from 3 to 30 seconds, and the duration should be adjusted based on the specific type of tissue and the thickness of the sections.
Precautions
1. Store in a sealed container. Once opened, use it as soon as possible, as its active ingredients are volatile.
2. For your safety and health, wear a lab coat and disposable gloves during operation.
3. This product is for research use only. Any other uses are strictly prohibited.