MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yuteng Hang,Rusha Gopali,Xiaorong Mao,Ling Zhu,Mengyang Tao,Guangjie Gu,Feng Li,Zhangxuan Liu,Xinnan Li,Ruyu Jiang,Xinxin Xu,Ning Bao,Yang Liu
Elevated cholesterol is a major cardiovascular risk factor, but current detection methods are resource-intensive. Non-invasive point-of-care testing is promising yet limited by sensitivity, and the blood-saliva cholesterol correlation requires further investigation. We developed an electrochemical biosensor featuring cholesterol oxidase immobilized on screen-printed carbon electrodes (SPCE) modified with conductive Au-Ag@Au core-shell nanoparticles and Prussian blue/poly-3,4-ethylenedioxythiophene (PB-PEDOT) nanocomposites. Morphological, spectroscopic, and electrochemical analyses (including impedance spectroscopy, heterogeneous electron transfer kinetics, and active surface area quantification) confirmed the biosensor superior conductivity. The biosensor achieved exceptional performance: a broad linear range (0.01–1.0 mM), low detection limit (3.3 μM amperometric LOD; 2.8 μM via EIS), and high sensitivity (57.3 μA/mM·cm 2 amperometric; 781 Ω/mM·cm 2 impedimetric). Clinical validation demonstrated excellent agreement with standard colorimetric methods in human serum and saliva for cholesterol tested in biological compounds and robust stability. A strong serum-saliva correlation (Pearson's correlation coefficient as 0.928) supports noninvasive monitoring feasibility. The anti-interference capability of the proposed biosensor was evaluated against common interferents, including glucose, lactic acid, uric acid, ascorbic acid, bovine serum albumin, cysteine, and their mixture. The results confirmed its high specificity against these typical biological matrix components. Particularly significant is its non-invasive detection capability, which eliminates the need for painful blood draws or invasive sample collection procedures. A key innovation lies in the synergistic integration of conductive Au-Ag@Au core-shell nanoparticles with PB-PEDOT nanocomposites on SPCEs, which significantly enhances electron transfer and catalytic activity, yielding an exceptionally sensitive and stable sensing platform. The biosensor sets itself apart by achieving superior analytical performance concurrently with robust clinical validation in both serum and saliva, firmly establishing its potential for reliable non-invasive cholesterol monitoring.
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FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Wenyue Wang,Xuan Li,Zhongxiang Fang,Jinfeng Bi
Pectin structure plays a vital role in determining the quality of food systems. This study investigated the impact of pectin structural variations on polyphenol oxidase (PPO)-catalyzed catechin oxidation using a series of pectin fractions prepared through polygalacturonase (PG) treatment. PG progressively degraded homogalacturonan (HG) regions, reduced molecular weight, and increased the exposure of hydroxyl and carboxyl groups, thereby converting the pectin matrix from a compact to a disordered state. Among these fractions, pectin enriched in rhamnogalacturonan I (RG-I) with an extended conformation, characterized by longer RG-I chains and fewer branches, exhibited weaker interactions with PPO and catechin, resulting in disordered aggregation and the lowest catalytic efficiency. Integration with molecular docking further revealed that high-HG pectin formed compact hydrophobic networks that restricted substrate access, resulting in high Vmax but low affinity. Conversely, low-HG pectin promoted hydrogen bonding with PPO and catechin, as well as facilitated ordered aggregation. Despite a lower Vmax, it exhibited a markedly reduced Km, resulting in enhanced overall catalytic efficiency. These findings demonstrate that pectin modulates PPO-catalyzed oxidation through steric hindrance, affinity interactions, and aggregation behavior, providing new insights for controlling enzymatic browning in food systems through structural modulation of pectin.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Junyan Yao,Bin Wang,Yanbin Zhao,Yingxi Xu,Cheng Wang,Jing Bai,Feng Xue,Paul K. Chu,Chenglin Chu
Although magnesium phosphate cement (MPC) has good biocompatibility and degradability, its poor compressive strength and insufficient osteogenic capacity hamper clinical applications. In this study, ultrasound-responsive drug-loading nanocapsules composed of melatonin-containing mesoporous silica and a layer of Pluronic F127 and hyaluronic acid (HA) are incorporated into the cement to enhance the mechanical and biological properties. The melatonin release rate, which can be controlled by the ultrasound intensity and time, reaches 72.45 ± 1.57 % after 7 days at 2 W/cm 2 . The simulated drug release may be based on reversible cleavage and polymerization of F127 under the cavitation effect of ultrasound. The modified cement exhibits a lower reaction temperature (31.41 ± 0.79 °C), longer solidification time (11.9 ± 0.78 min), higher compressive strength (30.19 ± 0.78 MPa), better injectability (90.36 ± 4.01 %), and improved degradation characteristics than the pure MPC due to the complexation effect and adsorption action of HA during hydration. In vitro experiments confirm the excellent antibacterial properties, cytocompatibility, as well as osteogenic capacity. The results reveal an effective strategy to develop multifunctional cement for orthopedic applications.
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MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Liangyin Shu,Liangjun Guan,Jinyi Tan,Hanting Li,Kailin Li,Jin Huang,Yufeng Zou,Qiru Chen,Huanting Li,Bowen Gao,Wei Cai,Shunli Xiao
The application of Natural Deep Eutectic Solvents (NADES) as a sustainable alternative to conventional organic solvents for extraction of bioactive compounds from traditional Chinese medicine (TCM) has gained considerable attention. However, challenges remain in removal of NADES and the recovery of extracted products. This study developed an efficient and eco-friendly ultrasonic-assisted NADES method for the extraction and enrichment of chlorogenic acid components from Duhaldea nervosa (DN). Fifteen NADES types were synthesized and evaluated, among which a mixture of choline chloride and 1,3-butanediol at a molar ratio of 1:6 was identified as optimal. Extraction conditions were optimized using Box-Behnken design combined with Response Surface Methodology (RSM), yielding the following parameters: 64 % NADES content, 11 mL solvent per gram of dry matter, and ultrasonic power of 300 W. The NKA-II resin demonstrated high efficacy in adsorbing and desorbing chlorogenic acids, with 40 % ethanol selected as the optimal eluent. This approach enabled efficient NADES removal and recovery of chlorogenic acids. The ultrasonic-enhanced continuous extraction and enrichment technology within the NADES system offers notable advantages including operational simplicity, environmental friendliness, cost-effectiveness, and high efficiency. A total of 55 chlorogenic acid components in the product obtained from the optimal extraction and enrichment process of DN were identified using feature-based molecular networking analysis. Furthermore, the effects of both the crude ethanol extract of DN and the chlorogenic acid-enriched product on RAW264.7 cell viability and lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophages were systematically evaluated.
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MATERIALS & DESIGN
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2025
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IF 7.9
Jingmei Huang,Ning Wang,Xiaoxiao Huang,Shihong Huang,Guoliang Xie,Jianfeng Zhang,Zhengzhao Li
Traumatic brain injury (TBI) is characterized by oxidative stress, neuroinflammation, and microglial dysregulation, leading to secondary neuronal damage. Here, we constructed coordination polymer nanoenzymes (Fe-FN CPs) with multiple enzyme-like activities and favorable biocompatibility. In vitro, Fe-FN CPs suppressed ROS accumulation, reduced pro-inflammatory cytokine release, and promoted microglial polarization toward the M2 phenotype. In vivo, Fe-FN CPs enhanced antioxidant defense, alleviated mitochondrial damage and cerebral edema, restored blood–brain barrier integrity, and improved cognitive performance. Mechanistically, Fe-FN CPs achieved dual regulation by inhibiting pro-inflammatory M1 polarization while promoting anti-inflammatory M2 polarization. These findings demonstrate that Fe-FN CPs exert potent neuroprotective effects and provide a promising nanomedicine strategy for TBI therapy based on natural active compounds.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Zhining Huang,Mengfan Hu,Jie Xu,Yana Chen,Tingzheng Shen,Xiaochao Li,Shaoju Fu
Smart magnetic textiles with multi-responsive characteristics, are promising building blocks for the flexible electronics areas of actuator, soft robot, and carrier rocket, etc. However, the fabrication of magnetic textiles with outstanding electromagnetic performances still remains great challenges. Here, smart magnetic three-dimensional (3D) textiles fabricated from natural macromolecule-based fibers composed of carboxymethyl cellulose (CMC) and sodium alginate (SA) contain Fe 3 O 4 knitting fabric, Ppy coating layer, and PAN/BaSO 4 electrospinning films were proposed, and their multi-functional characteristics were also fully evaluated. Notably, the magnetic textiles exhibited the relatively good radiative cooling effect under 100 μW/cm 2 light radiation encapsulation, and a reduction of 4.2 °C compared to that of the fabric before encapsulation, the multi-responsive characteristics including sensitivity, magnetic driving performances, and electromagnetic shielding property were good presented, and the cell phone radiation could be reduced from 57.22 μW/cm 2 to 10.745 μW/cm 2 for incoming signals. This study employed an efficient and environmentally friendly fabricating strategy for magnetic textiles with multi-responsive characteristics, showing great potential for the flexible electronic soft material application.
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PubMed
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Materials Horizons
·
2025
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IF 10.7
Ping Wu,Xufei An,Bohui Zheng,Wuque Wang,Kexuan Wang,Hanbin Liu,Yao Yao,Hongwei Zhou
Ionically conductive hydrogels (ICHs) are increasingly explored in flexible electronic devices but the mechanoelectrical and electrochemical performances of hydrogel devices are often limited. Herein, bubble-mediated pores are established inside ICHs to achieve high sensitivity in pressure sensors and high-performance in rechargeable zinc-ion hybrid supercapacitors containing ICHs as electrolytes. CO2 bubbles generated from an embedded reaction between weak acids and bases are trapped within the viscous polyvinyl alcohol/poly (sodium acrylic acid) (PVA/PAANa) pre-gel solution as a soft template. Cyclic freezing–thawing treatment of pre-gel solutions results in fully physically cross-linked ICHs containing pores that are regulated by acid–base reactions. Interestingly, by regulating the feed amount of acids and bases, porous channels are obtained in ICHs due to the orientation of rising bubbles in the precursor solution. Because of the synergistic capacitance effect from high-specific-area pores, the sensitivity of capacitive pressure sensors containing porous ICHs as electrodes is improved by 10 times. Additionally, porous ICHs also promisingly work as electrolytes in zinc-ion hybrid supercapacitors. Stable charge–discharge in nearly 30 000 cycles with high coulombic efficiency (∼100%) has been achieved. The bubble-mediated method is expected to provide a new pathway to improve the performance of hydrogel-based devices through structural regulation.
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PubMed
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Nanoscale
·
2025
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IF 5.1
Shenglong Wu,Lixiong Du,Yajing Wang,Kui Shen,Liyu Chen,Yingwei Li
The reverse water-gas shift (RWGS) reaction provides an important pathway for converting CO2 to CO, which can then be used to produce high-value chemicals through Fischer-Tropsch synthesis. Metal oxide materials, especially Fe2O3, offer considerable potential for use in RWGS reaction at high temperatures, but their catalytic performance is hindered by the inadequate activation and dissociation capabilities of H2 at low temperatures. Herein, we report the introduction of Fe4 with high H2 activation capability near Fe2O3, which shows a synergistic effect and achieves excellent activity and selectivity in RWGS reaction at low temperatures. Fe2O3 nanoparticles and Fe4 clusters supported on carbon (Fe2O3-Fe4/C) were prepared by KCl-assisted pyrolysis of NH2-MIL-88B(Fe) precursors. Fe2O3-Fe4/C delivers a remarkable CO selectivity of 99.4% and a space-time yield of 15.2 μmolCO gcat−1 s−1 at 350 °C and atmospheric pressure. In situ diffuse reflection infrared Fourier-transform spectra and density functional theory calculations demonstrate that the introduction of Fe4 clusters can activate H2 and significantly lower the reaction energy barrier of CO2 hydrogenation on Fe2O3 sites, thus boosting the RWGS performance at low temperatures.
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Journal of Energy Chemistry
·
2025
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IF 14.9
Tongzhou Li,Xiao Lin,Jingchen Na,Taipu Chen,Hongjie Zhang,Zhiqiang Wang,Longsheng Cao,Liang Hong,Zhigang Shao,Wen-Feng Lin
Large-scale hydrogen production via water electrolysis faces a freshwater shortage. Direct seawater electrolysis offers a solution but encounters new challenges. Herein, we report a feasible strategy to both prevent metal hydroxide deposition and boost the hydrogen evolution reaction by adding a chelating agent, EDTA-Na 4 , that chelates with Mg 2+ /Ca 2+ , thus inhibiting their deposition and gathering them near the cathode surface, resulting in breaking the ordered hydrogen bond networks of interfacial water and reducing the activation energy of water dissociation. Furthermore, hydrolysis of –COO − also promoted water dissociation to produce more active *H and *OH near the electrode surface that in turn serves as a diffusion medium for *OH, accelerating mass transfer and enabling seawater electrolysis to exhibit a stable performance, which operates continuously at 100 mA cm −2 @2.20 V and 200 mA cm −2 @2.58 V for 400 h in the symmetric electrolyzer and 500 mA cm −2 @2.29 V for over 500 h in the asymmetric electrolyzer. This study provides a new perspective to address the issues of stable and scalable direct seawater electrolysis for practical green hydrogen production.
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INORGANICA CHIMICA ACTA
·
2025
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IF 3.2
Qian Sun,Lu Ren,Jing Wang,Kun Bai,Chang Liu,Yanqi Liu,Dawei Zhang
Coumarin-structured ( E )-7-(diethylamino)-3-(1-(2-(4-methylbenzo[ d ]thiazol-2- yl)hydrazono)ethyl)-2 H -chromen-2-one (HTM) was synthesized in two steps using N , N -diethylsalicylaldehyde, ethyl acetoacetate and 4-methyl-2-hydrazine-benzothiazole as raw materials. The HTM was characterized by FT-IR, 1 H NMR, 13 C NMR and HR-MS. It was found that the probe HTM has rapid and highly selective recognition ability for Cu 2+ in EtOH/H 2 O (1:1, v /v). The spectral characteristics of the probe HTM were investigated using fluorescence spectroscopy and ultraviolet-visible spectroscopy. The results showed that the binding of probe HTM to Cu 2+ produced a significant fluorescence quenching effect, and the influence of other metal ions was negligible. In visible light, when HTM interacts with Cu 2+ , the solution changes from yellow to orange as observed by the naked eye. Subsequently, the binding mechanism of the probe HTM to Cu 2+ was explored by Job's plot, 1 H NMR titration and DFT calculations, and it was found that they cooperated in a 1:1 manner. Linear equations for ultraviolet-visible and fluorescence intensities at different Cu concentrations were fitted, and the detection limits (LOD) were calculated to be 5.22 nM and 3.70 nM, respectively. Experimental studies on the recovery rates of standard additions indicated that the probe HTM could be used for the quantitative detection of Cu 2+ in actual water samples. The potential application of this probe in fingerprint detection was also investigated. In addition, the probe has been successfully applied to Cu 2+ monitoring in living cells.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Keqing Wang,Yujie Hao,Yaru Duan,Xueqian Guo,Zhenyue Yang,Pierre Waffo-Téguo,Michaël Jourdes,Pierre-Louis Teissedre,Wen Ma
Esterified flavan-3-ols, prevalent in grapes and wine as monomers or subunits within condensed tannins, significantly influence the mouthfeel of wine. However, comprehensive knowledge of their molecular structures and transformations during vinification is still limited. This study investigated tannin derivatives, specifically vanillate and gallate forms, using UHPLC-Q-TOF to elucidate their distribution across four Vitis vinifera L. cultivars, their transformations during vinification, and their metabolic fate mediated by Oenococcus oeni and β -glucosidase activity. The taste responses of epicatechin-3- O -vanillate (ECV) and − 3- O -gallate (ECG) were evaluated using an electronic tongue. The results indicated that ECV was predominantly found as monomers and terminal units within the condensed tannins across all examined grape varieties. During early malolactic fermentation (MLF), ECV and ECG are hydrolyzed into epicatechin (EC), vanillic acid (VA), and gallic acid (GA) by O. oeni and β -glucosidase enzymes. This enzymatic cleavage is potentially correlated with reduction of esterified flavan-3-ols contentand perceptible bitterness in wine.
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PubMed
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POLYMER TESTING
·
2025
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IF 6
Yingying Zhang,Cancan Yang,Chongyue Bai,Xuanye Wang,Chao Feng,Huige Wei
Polyacrylamide (PAM) based hydrogels are promising for soft strain sensor applications, while the incorporation of vinylimidazole (VI) endows the hydrogels with unique ion responsiveness and good biocompatibility. In this study, a high-performance PAM-co-VI/Ni/Co hydrogel was successfully fabricated through copolymerization of PAM with VI followed by modification with bimetallic nickel-cobalt ions. Experimental results demonstrate that when the VI content is 30% with a Ni/Co ion ratio of 3:1, the hydrogel exhibits remarkable comprehensive properties, with a tensile fracture strength of 0.46 MPa, an ultrahigh fracture strain of 1800%, a tensile sensitivity of 4.62, and long-term durability exceeding 1000 cycles. Furthermore, the hydrogel shows high pressure sensitivity (0.059 kPa -1 in the 0-5 kPa range), enabling precise detection of various mechanical signals from acoustic vibrations to complex human motions. Owing to its superior conductivity and biocompatibility, the PAM-co-VI/Ni/Co hydrogel also functions as a flexible electrode for electrocardiogram (ECG) monitoring, achieving conformal skin contact while stably acquiring high signal-to-noise ratio ECG waveforms with accurate identification of P-waves, Q, R, and S waves (QRS complexes), and other key features, demonstrating great potential for wearable health monitoring applications.
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